Development of high-throughput measurement techniques of pharmacokinetics using stigmatic imaging mass spectrometry

2012 Fiscal Year Final Research Report

• Project/Area Number: 23710271
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemical biology
• Research Institution: Osaka University
• Principal Investigator: HAZAMA Hisanao 大阪大学, 大学院・工学研究科, 助教 (70437375)
• Research Collaborator: MORIGUCHI Naoki 大阪大学, 大学院・工学研究科, 博士前期課程 ; HAMANAKA Tomonori 大阪大学, 工学部 ; KANNNEN Hiroki 大阪大学, 工学部
• Project Period (FY): 2011 – 2012
• Keywords: 分子イメージング / 薬物動態 / イメージング質量分析 / 投影型 / マトリックス支援レーザー脱離イオン化

Research Abstract

Technical developments were performed for high-throughput measurement of pharmacokinetics using stigmatic imaging mass spectrometry withmatrix-assisted laser desorption/ionization. Optimum ionization conditions for the drug were investigated, and a sample translation device operating under high vacuum was developed. The spatial distribution of the drug in a brain of a mouse was successfully observed by using the investigated ionization conditions and the improved mass spectrometer.

Research Products

• [Journal Article] Effect of solvent on ionization efficiency in matrix-assisted laser desorption/ionization mass spectrometry of peptides (2013)
  • Author(s): Hisanao Hazama, Satoshi Furukawa, and Kunio Awazu
  • Journal Title: Chemical Physics Volume: (in press)
  • DOI: DOI:10.1016/j.chemphys.2013.03.004
  • Peer Reviewed
• [Journal Article] Theoretical analysis of correlation between ionization threshold fluence in IR-MALDI and IR absorption spectrum of matrix molecules (2013)
  • Author(s): Makoto Hatakeyama, Takako Mashiko, Hisanao Hazama, Kunio Awazu, and Masanori Tachikawa
  • Journal Title: International Journal of Quantum Chemistry Volume: 113(2) Pages: 125-129
  • DOI: DOI:10.1002/qua.24296
  • Peer Reviewed
• [Journal Article] がん治療薬剤の動態解析に向けたレーザーイオン化イメージング質量分析手法の開発 (2013)
  • Author(s): 閑念弘樹, 守口直樹, 間久直, 波多野浩士, 金田安史, 藤野竜也, 粟津邦男
  • Journal Title: 電気学会 光・量子デバイス研究会資料(バイオメディカ ルフォトニクス応用) Volume: OQD-13-017 Pages: 27-32
• [Journal Article] 薬剤のレーザーイオン化質量分析イメージングにおけるゼオライトのイオン化補助効果 (2012)
  • Author(s): 守口直輝, 間久直, 藤野竜也, 粟津邦男
  • Journal Title: 電気学会 光・量子デバイス研究会資料(バイオメディカルフォトニクス応用) Volume: OQD-12-027 Pages: 11-15
• [Journal Article] Development of a stigmatic mass microscope for high-throughput molecular imaging (2012)
  • Author(s): Hisanao Hazama, Tomonori Hamanaka, and Kunio Awazu
  • Journal Title: Proceedings of Conference on Laser Surgery and Medicine 2012 Pages: 80-81
• [Journal Article] レーザーイオン化質量分析での薬剤イメージングに向けた試料作製条件と画像処理法の検討 (2012)
  • Author(s): 浜中智紀, 間久直, 粟津邦男
  • Journal Title: 電気学会 光・量子デバイス研究会資料(バイオメディカルフォトニクス応用) Volume: OQD-12-022 Pages: 15-20
• [Journal Article] Tissue imaging with a stigmatic mass microscope using laser desorption/ionization (2012)
  • Author(s): Kunio Awazu, Hisanao Hazama, Tomonori Hamanaka, Jun Aoki, Michisato Toyoda, and Yasuhide Naito
  • Journal Title: Proceedings of SPIE Volume: 8225 Pages: 82252E
  • DOI: DOI:10.1117/12.909898
• [Journal Article] 投影型イメージング質量分析装置の開発 (2011)
  • Author(s): 間久直, 粟津邦男
  • Journal Title: 生産と技術 Volume: 63(4) Pages: 43-51
• [Presentation] Imaging mass spectrometry of pharmaceutical drugs using a high mass resolution MALDI-SpiralTOF (2013)
  • Author(s): Takaya Satoh, Ayumi Kubo, Jun Tamura, Carlos Chiu, Naoki Moriguchi, Hisanao Hazama, Kunio Awazu, and Michisato Toyoda
  • Organizer: 4th Asia Oceania Mass Spectrometry Conference and 10th Taiwan Society for Mass Spectrometry Annual Conference
  • Place of Presentation: Taipei International Convention Center, Taipei, Taiwan
  • Year and Date: 20130710-12
• [Presentation] Development of new stigmatic imaging mass spectrometer and its application to metal cation distribution in fish (2013)
  • Author(s): Jun Aoki, Hisanao Hazama, Kunio Awazu, and Michisato Toyoda
  • Organizer: 61st ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Minneapolis Convention Center, Minneapolis, MN, USA
  • Year and Date: 2013-06-13
• [Presentation] Imaging mass spectrometry of pharmaceutical drugs using a high mass resolution MALDI-SpiralTOF-TOF (2013)
  • Author(s): Ayumi Kubo, Takaya Satoh, Robert A. DiPasquale, Naoki Moriguchi, Hisanao Hazama, Kunio Awazu, and Michisato Toyoda
  • Organizer: 61st ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Minneapolis Convention Center, Minneapolis, MN, USA
  • Year and Date: 2013-06-12
• [Presentation] 投影型顕微イメージング質量分析装置による生体試料の観察 (2013)
  • Author(s): 間久直, 青木順、豊田岐聡、内藤康秀, 粟津邦男
  • Organizer: 電気学会第1回量子ビームによるナノバイオサイエンス技術調査専門委員会
  • Place of Presentation: 大阪大学産業科学研究所, 大阪府吹田市
  • Year and Date: 2013-05-08
• [Presentation] がん治療薬剤の動態解析に向けたレーザーイオン化イメージング質量分析手法の開発 (2013)
  • Author(s): 閑念弘樹, 守口直樹, 間久直, 波多野浩士,金田安史, 藤野竜也, 粟津邦男
  • Organizer: 電気学会 光・量子デバイス研究会(バイオメディカルフォトニクス応用)
  • Place of Presentation: 東北大学東京分室, 東京都千代田区
  • Year and Date: 2013-03-04
• [Presentation] レーザーイオン化質量分析装置を用いた薬剤イメージングにおけるゼオライトのイオン化補助効果の検討 (2013)
  • Author(s): 守口直輝, 間久直, 藤野竜也, 粟津邦男
  • Organizer: レーザー学会学術講演会第33回年次大会
  • Place of Presentation: 姫路商工会議所, 兵庫県姫路市
  • Year and Date: 2013-01-30
• [Presentation] Novel stigmatic mass microscope using laser ionization (2012)
  • Author(s): Hisanao Hazama and Kunio Awazu
  • Organizer: International Joint Symposium on Single-Cell Analysis (The 6th International Workshop on Approaches to Single-Cell Analysis & The 8th International Forum on Post-Genome Technologies)
  • Place of Presentation: Kyoto Research Park, Kyoto, Japan
  • Year and Date: 2012-11-27
• [Presentation] 薬剤のレーザーイオン化質量分析イメージングにおけるゼオライトのイオン化補助効果 (2012)
  • Author(s): 守口直輝, 間久直, 藤野竜也, 粟津邦男
  • Organizer: 電気学会 光・量子デバイス研究会(バイオメディカルフォトニクス応用)
  • Place of Presentation: 東北大学 東京分室 , 東京都千代田区
  • Year and Date: 2012-09-24
• [Presentation] Development and applications of new stigmatic mass microscope with high mass and spatial resolving power using multi-turn time-of-flight mass spectrometer (2012)
  • Author(s): Jun Aoki, Hisanao Hazama, Kunio Awazu, and Michisato Toyoda
  • Organizer: 19th International Mass Spectrometry Conference (IMSC 2012)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-09-19
• [Presentation] Imaging mass spectrometry and structural analysis of lipids directly on tissue specimens using a high resolution MALDI-TOF-TOF tandem mass spectrometer (2012)
  • Author(s): Shuichi Shimma, Ayumi Kubo, Takaya Satoh, Hisanao Hazama, Kunio Awazu, and Michisato Toyoda
  • Organizer: 19th International Mass Spectrometry Conference (IMSC 2012)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-09-19
• [Presentation] Investigation of zeolite matrix for analysis of pharmacokinetics using imaging mass spectrometry with laser desorption/ionization (2012)
  • Author(s): Naoki Moriguchi, Hisanao Hazama, Tatsuya Fujino, and Kunio Awazu
  • Organizer: 19th International Mass Spectrometry Conference (IMSC 2012)
  • Place of Presentation: Kyoto International Conference Center, Kyoto, Japan
  • Year and Date: 2012-09-17
• [Presentation] Development of a stigmatic mass microscope for high-throughput molecular imaging (2012)
  • Author(s): Hisanao Hazama, T omonori Hamanaka, and Kunio Awazu
  • Organizer: Conference on Laser Surgery and Medicine 2012 (CLSM 2012)
  • Place of Presentation: Pacifico Yokohama, Kanagawa, Japan
  • Year and Date: 2012-04-27
• [Presentation] レーザーイオン化質量分析での薬剤イメージングに向けた試料作製条件と画像処理法の検討 (2012)
  • Author(s): 浜中智紀, 間久直, 粟津邦男
  • Organizer: 電気学会 光・量子デバイス研究会資料(バイオメディカルフォトニクス応用)
  • Place of Presentation: 東北大学 東京分室 , 東京都千代田区
  • Year and Date: 2012-03-05
• [Presentation] Tissue imaging with a stigmatic mass microscope using laser desorption/ionization (2012)
  • Author(s): Kunio Awazu, Hisanao Hazama, T omonoriHamanaka, Jun Aoki, Michisato Toyoda, and Y asuhide Naito
  • Organizer: SPIE Photonics West 2012
  • Place of Presentation: BiOS, The Moscone Center, San Francisco, CA, USA
  • Year and Date: 2012-01-23
• [Presentation] レーザー技術が拓く新規バイオ・医療応用 (2011)
  • Author(s): 間久直, 粟津邦男
  • Organizer: 第2回レーザー共同研究所成果報告会
  • Place of Presentation: 日本原子力研究開発機構敦賀本部, 福井県敦賀市
  • Year and Date: 2011-12-01
• [Presentation] レーザーイオン化投影型質量顕微鏡による生体組織切片の観察 (2011)
  • Author(s): 間久直, 浜中智紀, 青木順, 豊田岐聡, 内藤康秀, 粟津邦男
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク ,大阪府吹田市
  • Year and Date: 2011-09-14
• [Presentation] 高空間分解能・高質量分解能の投影式多重周回飛行時間型イメージング質量分析装置の開発 (2011)
  • Author(s): 青木順, 豊田岐聡, 間久直, 粟津邦男, 藤井研一, 内藤康秀
  • Organizer: 第59回質量分析総合討論会
  • Place of Presentation: ホテル阪急エキスポパーク,大阪府吹田市
  • Year and Date: 2011-09-14
• [Presentation] Observation of tissue sections using a stigmatic mass microscope with a multi-turn time-of-flight mass spectrometer (2011)
  • Author(s): Hisanao Hazama, Hidetoshi Yoshimura, Jun Aoki, Hirofumi Nagao, Y asuhide Naito, Michisato Toyoda, Katsuyoshi Masuda, Kenichi Fujii, Toshio Tashima, and Kunio Awazu
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2011-06-07
• [Presentation] Development and evaluation of new stigmatic mass microscope with high mass and spatial resolving power using multi-turn time-of-flight mass spectrometer (2011)
  • Author(s): Jun Aoki, Hisanao Hazama, MichisatoToyoda, Kunio Awazu, Katsuyoshi Masuda, Kenichi Fujii, and Y asuhide Naito
  • Organizer: 59th ASMS Conference on Mass Spectrometry and Allied Topics
  • Place of Presentation: Colorado Convention Center, Denver, CO, USA
  • Year and Date: 2011-06-07
• [Book] 日本臨床プロテオーム研究会編, "臨床プロテオミクス-バイオマーカー探索から個別化医療へ-,"、8-3 "新規 MS イメージング技術," (2012)
  • Author(s): 間久直, 粟津邦男
  • Total Pages: 299-308
  • Publisher: 金原出版
• [Book] レーザー学会編, "先端固体レーザー,"、第9 章 "レーザー医用応用 ," (2011)
  • Author(s): 石井克典, 間久直, 粟津邦男
  • Total Pages: 371-408
  • Publisher: オーム社