2012 Fiscal Year Final Research Report
Development ofa novel method for analyzing various DNA methylation
| Project/Area Number |
23750083
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Analytical chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
SUZUKI Hirokazu 九州大学, 大学院・農学研究院, 准教授 (80462696)
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| Project Period (FY) |
2011 – 2012
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| Keywords | メチル化塩基 / メチル化部位分析 / 抗体 |
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| Research Abstract |
Microbes harbor various methylated DNA species, such as 5-methylcytosine, N6-methyladenine, and N4-methylcytosine, which are produced by DNA restriction-modification systems. Aiming at the development of a novel method for widely analyzing DNA methylation sites, I designed immunological methods to analyze N6-methyladenine sites arising from type I restriction-modification systems (immunoprecipitation/PCR, methylated plasmid library/HPLC, methylated plasmid library/western blot) and evaluated them. Detailed analysis suggested that the method “methylated plasmid library/western blot” was effective for analyzing type I methylation sites.
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