Analysis of cellular functions in primary cells usingscanning electrochemical microscopy

2012 Fiscal Year Final Research Report

• Project/Area Number: 23750095
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Analytical chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: HIRANO Yu 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (70415735)
• Project Period (FY): 2011 – 2012
• Keywords: 生体分析

Research Abstract

Analysis of primary-cell culture is currently used to evaluate the pharmacological and toxicological properties of drugs. We developed a SECM system for analyzing contraction kinetics in cardiomyocytes and cellular volume in adipocyte. This system enabled

Research Products

• [Journal Article] Development of a scanning electrochemical microscopy-based micropipette and its application toanalysis of topographic change of single-cell (2013)
  • Author(s): Y. Hirano, K. Kowata, M. Kodama, Y. Komatsu
  • Journal Title: Bioelectrochemistry Volume: 92 Pages: 1-5
  • DOI: DOI:10.1016/j.bioelechem.2013.01.004
  • Peer Reviewed
• [Journal Article] Imaging of Enzyme Reactions Captured via Immuno-recognition by Scanning Electrochemical Microscopy with Distance Control System (2012)
  • Author(s): Y. Hirano, T. Yasukawa, Y. Mase, D. Oyamatsu, H. Shiku, F. Mizutani and T. Matsue
  • Journal Title: Electrochemistry Volume: 80 Pages: 30-32
  • DOI: DOI:10.5796/electrochemistry.80.30
  • Peer Reviewed
• [Presentation] Electrochemical monitoring of enzyme reactions promoted on double stranded DNA (2011)
  • Author(s): Y. Hirano, K. Ichikawa, N. Kojima, and Y. Komatsu
  • Organizer: 第38回国際核酸化学シンポジウム
  • Place of Presentation: 北海道大学(北海道)
  • Year and Date: 2011-11-10