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2012 Fiscal Year Final Research Report

An advanced assay system for stress hormone-like chemicals in cardiac tissues

Research Project

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Project/Area Number 23750096
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Analytical chemistry
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

KIM Sung-bae  独立行政法人産業技術総合研究所, 環境管理技術研究部門, 主任研究員 (60470043)

Project Period (FY) 2011 – 2012
Keywords生物発光酵素 / 一分子型生物発光プローブ / ストレスホルモン / イメージング / 超高輝度 / バイオ診断 / 心筋組織分化 / 高輝度化
Research Abstract

The present study was purposed to construct an advanced assay system for stress hormone-like chemicals in mammalian cells and cardiac tissues. In respect to the purpose, I conducted a variety of studies ranging from creation of new artificial luciferases

  • Research Products

    (30 results)

All 2013 2012 2011

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (14 results) Book (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 1 results)

  • [Journal Article] A Bioluminescent Assay System for Whole-Cell Determination of Hormones2013

    • Author(s)
      Sung Bae Kim, Toshiyuki Suzuki b, and Akira Kimura
    • Journal Title

      Chemical and Pharmaceutical Bulletin

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Molecular Imaging Probes Based on Bioluminescence and Fluorescence2013

    • Author(s)
      Sung Bae Kim and Ryuichi Naganawa
    • Journal Title

      Bunseki Kagaku

      Volume: (in press) Pages: 62(5)

    • Peer Reviewed
  • [Journal Article] Advances in Fluorescence and Bioluminescence Imaging2013

    • Author(s)
      Takeaki Ozawa, Hideaki Yoshimura, Sung Bae Kim
    • Journal Title

      Analytical Chemistry

      Volume: 85 Pages: 590-609

    • Peer Reviewed
  • [Journal Article] Intelligent Design of Nano-Scale Molecular Imaging Agents2012

    • Author(s)
      Sung Bae Kim, Mitsuru Hattori, and Takeaki Ozawa
    • Journal Title

      International Journal of Molecular Science

      Volume: 13 Pages: 16986-17005

  • [Journal Article] Correction to Superluminescent variants of marine luciferases for bioassays2012

    • Author(s)
      Sung Bae Kim, Hideyuki Suzuki, Moritoshi Sato, Hiroaki Tao
    • Journal Title

      Analytical Chemistry

      Volume: 84 Pages: 4244-4244

  • [Journal Article] Bioluminescent Capsules for Live-Cell Imaging2012

    • Author(s)
      Sung Bae Kim, Yuzuru Ito, Masaki Torimura
    • Journal Title

      Bioconjugate Chemistry

      Volume: 23 Pages: 2221-2228

  • [Journal Article] Labor-effective manipulation of marine and beetle luciferases for bioassays2012

    • Author(s)
      Sung Bae Kim
    • Journal Title

      Protein Engineering, Design, & Selection

      Volume: 25(6) Pages: 261-269

    • Peer Reviewed
  • [Journal Article] Superluminescent variants of marine luciferases for bioassays2011

    • Author(s)
      Sung Bae Kim, Hideyuki Suzuki, Moritoshi Sato, Hiroaki Tao
    • Journal Title

      Analytical Chemistry

      Volume: 83 Pages: 8732-8740

    • Peer Reviewed
  • [Journal Article] A bioluminescent probe for salivary cortisol2011

    • Author(s)
      Sung Bae Kim, Y Takenaka, M Torimura
    • Journal Title

      Bioconjugate Chemistry

      Volume: 22 Pages: 1835-1841

    • Peer Reviewed
  • [Journal Article] 分子イメージング用生物発光プローブの開発2011

    • Author(s)
      金 誠培, 博明 田尾
    • Journal Title

      進歩総説「ぶんせき」誌

      Volume: 2011 年 3 月号 Pages: 150-158

    • Peer Reviewed
  • [Presentation] 科学が拓く産業技術にふれてみる‐生物発光の不思議発見2013

    • Author(s)
      金 誠培
    • Place of Presentation
      サイエンスキャンプ(JST 主催)、産総研さくら館
    • Year and Date
      20130109-11
  • [Presentation] 生物発光計測デバイスの開発と応用2013

    • Author(s)
      金 誠培
    • Organizer
      日本化学会第93回年会
    • Place of Presentation
      立命館大学びわこキャンパス, ATP ポスター発表
    • Year and Date
      2013-03-23
  • [Presentation] 生物発光カプセルの開発と応用2013

    • Author(s)
      金 誠培
    • Organizer
      日本化学会第93回年会
    • Place of Presentation
      立命館大学びわこキャンパス
    • Year and Date
      2013-03-23
  • [Presentation] 生物発光を用いたホルモンセンシング2013

    • Author(s)
      金 誠培
    • Place of Presentation
      センサ見本市、産総研共用講堂
    • Year and Date
      2013-01-21
  • [Presentation] ホルモン様化学物質診断技術の開発と応用2013

    • Author(s)
      金 誠培
    • Organizer
      E&E フォラム
    • Place of Presentation
      産総研共用講堂, ポスター発表
    • Year and Date
      2013-01-18
  • [Presentation] 生物発光カプセルの開発と細胞死イメージングへの展開2012

    • Author(s)
      金 誠培
    • Organizer
      日本生物発光化学発光研究会第29回学術講演会
    • Place of Presentation
      産総研臨海副都心センター, ポスター発表
    • Year and Date
      2012-11-17
  • [Presentation] Labor-effective manipulation of marine and beetle luciferases for bioassays2012

    • Author(s)
      金 誠培
    • Organizer
      日本分析化学会第61回年会
    • Place of Presentation
      金沢大学、口頭発表 C2009
    • Year and Date
      2012-09-20
  • [Presentation] A Bioluminescent Assay Device for Whole Cell Assays2012

    • Author(s)
      金 誠培
    • Organizer
      日本分析化学会第61回年会
    • Place of Presentation
      金沢大学、口頭発表 C2008
    • Year and Date
      2012-09-20
  • [Presentation] 高輝度生物発光プローブの開発とホルモンアッセイへの展開2012

    • Author(s)
      Sung-Bae Kim
    • Organizer
      平成 23 年産総研 環境・エネルギーシンポジウム
    • Place of Presentation
      「環境・ヘルスケアを支える将来の診断技術開発」機械振興会館、東京
    • Year and Date
      2012-08-30
  • [Presentation] 高輝度生物発光プローブの開発とバイオアッセイへの展開2012

    • Author(s)
      金 誠培
    • Organizer
      平成 23 年度産総研本格研究ワークショップin 東北
    • Place of Presentation
      仙台ガーデンパレス, ポスター発表
    • Year and Date
      2012-03-07
  • [Presentation] 分子発光プローブの設計とホルモン様化学物質計測への展開2011

    • Author(s)
      金 誠培
    • Place of Presentation
      産総研 2011 年度オープンラボ参加(出品)
    • Year and Date
      20111013-14
  • [Presentation] Bioluminescent indicators for illuminating steroid hormones2011

    • Author(s)
      Sung-Bae Kim, Hiroaki Tao
    • Organizer
      JAIMA Conference 2011、The Japan Society for Analytical Science
    • Place of Presentation
      Makuhari Messe, A048, Poster
    • Year and Date
      2011-09-08
  • [Presentation] Integrated-molecule-format multicolor probes for illuminating intracellular molecular events2011

    • Author(s)
      Sung Bae Kim
    • Organizer
      IUPAC International Congress on Analytical Sciences (ICAS) 2011
    • Place of Presentation
      Kyoto Intl Conference Center, ICAS11-P0212
    • Year and Date
      2011-05-25
  • [Presentation] Genetically encoded bioluminescent indicators for stress hormones2011

    • Author(s)
      Sung Bae Kim
    • Organizer
      IUPAC International Congress on Analytical Sciences (ICAS) 2011
    • Place of Presentation
      Kyoto Intl Conference Center, 24pD08 (ICAS11-P0211)
    • Year and Date
      2011-05-24
  • [Book] Fabrication of Bioluminescent Capsules and Live-Cell Imaging, MethodinMolecularBiology2013

    • Author(s)
      Sung Bae Kim
    • Publisher
      Springer - Humana Press(in Press)
  • [Book] Optical Assays for Determining Hormone Actions - Spying cell-to-cell communications, Nanotechnologyvol12Bioimaging2013

    • Author(s)
      Sung Bae Kim
    • Total Pages
      315-341
    • Publisher
      Studium Press LLC
  • [Patent(Industrial Property Rights)] バイオアッセイに最適な反応溶液の決定2012

    • Inventor(s)
      金 誠培(80%) 、中里 哲也(10%)、鳥村 政基(10%)
    • Industrial Property Rights Holder
      独)産業技術総合研究所
    • Industrial Property Number
      特願 2012-236872
    • Filing Date
      2012-10-26
  • [Patent(Industrial Property Rights)] 人工生物発光酵素(Artificial Luciferase; ALuc)2012

    • Inventor(s)
      金 誠培(80%) 、鳥村 政基(10%)、田尾 博明(10%)
    • Industrial Property Rights Holder
      独)産業技術総合研究所
    • Industrial Property Number
      特願 2012-237043
    • Filing Date
      2012-10-26
  • [Patent(Industrial Property Rights)] 発光測定デバイス2012

    • Inventor(s)
      金 誠培(80%) 、鈴木 俊幸 (10%)木村 明(10%)
    • Industrial Property Rights Holder
      独)産業技術総合研究所
    • Industrial Property Number
      特願 2012-179597
    • Filing Date
      2012-08-13
  • [Patent(Industrial Property Rights)] 多重認識型生物発光プローブシステム2011

    • Inventor(s)
      金 誠培(80%) , 竹中 康浩(10%) , 鳥村 政基(10%)
    • Industrial Property Rights Holder
      独)産業技術総合研究所
    • Industrial Property Number
      PCT/JP2011/080419
    • Filing Date
      2011-12-28
    • Overseas

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Published: 2014-09-25  

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