2013 Fiscal Year Final Research Report
Hydrated ionic liquid for dissolution and refolding of proteins
| Project/Area Number |
23750120
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Polymer chemistry
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
FUJITA Kyoko 東京農工大学, 工学(系)研究科(研究院), 講師 (90447508)
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| Project Period (FY) |
2011 – 2012
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| Keywords | イオン液体 / 生体高分子 |
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| Research Abstract |
Physicochemical property of hydrated ionic liquid which is composed by cholinium cation ([ch]) and phosphate anion ([dhp]) has been analyzed with changing the water contents. The water molecules were interacted with ions strongly and existed as bound water. Proteins and nucleobase pairs were dissolved in hydrated [ch][dhp] with remained the higher ordered structure. Furthermore, the activity of dissolved biomolecules in hydrated [ch][dhp] were observed by spectroscopically and electrochemically. Furthermore, thermal stability and long term stability of the responses has been improved in hydrated [ch][dhp].
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