Development of hyper signal amplification method by fluorogenic reaction of oligonucleotides

2012 Fiscal Year Final Research Report

• Project/Area Number: 23750178
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Chemistry related to living body
• Research Institution: Hokkaido University
• Principal Investigator: SATO Kousuke 北海道大学, 大学院・薬学研究院, 助教 (70415686)
• Project Period (FY): 2011 – 2012
• Keywords: 核酸 / 蛋白質 / 糖化学

Research Abstract

We synthesized a nucleoside phosphoramidite containing aaminothiophenol structure for cross-link with 5-formyl-2 ' -deoxyuridine.Oligodeoxyribonucleotide (ODN) were also synthesized by DNA synthesizer, and deprotectedand purified. The reaction between ODN containing aminothiophenol and 5-formy-2 ' -deoxyuridine was investigated on the complementary template ODN. We also designed thesequence of ODNs by computational modeling.

Research Products

• [Journal Article] Structure of the DNA (6-4) photoproduct dTT(6-4)TT in complex with the 64M-2 antibody Fab fragment implies increased antibody-binding affinity by the flanking nucleotides (2012)
  • Author(s): Yokoyama, H.; Mizutani, R.; Satow, Y.; Sato, K.; Komatsu, Y.; Ohtsuka, E.; Nikaido
  • Journal Title: O. Acta Cryst Volume: D68 Pages: 232-238
  • DOI: doi:10.1107/S0907444912000327
  • Peer Reviewed
• [Journal Article] Highly Fluorescent 5-(5,6-Dimethoxy- benzothiazol-2-yl)-2'-Deoxyuridine 5'-Triphosphate as an Efficient Substrate for DNA Polymerases (2011)
  • Author(s): Sato, K.; Sasaki, A.; Matsuda, A
  • Journal Title: ChemBioChem Volume: 12 Pages: 2341-2346
  • DOI: doi:10.1002/cbic.201100452
  • Peer Reviewed
• [Journal Article] Fluorescence Properties of 5-(5,6- Dimethoxybenzothiazol-2-yl)-2'-Deoxy-uridine (dbtU) and Oligodeoxy-ribonucleotides containing dbtU.Hirose (2011)
  • Author(s): W.; Sato, K.; Matsuda, A
  • Journal Title: Eur. J. Org. Chem Pages: 6206-6217
  • DOI: doi:10.1002/ejoc.201100818
  • Peer Reviewed
• [Journal Article] Highly efficient enzymatic synthesis of 3'-deoxyapionucleic acid (apioNA) having the four natural nucleobases (2011)
  • Author(s): Kataoka, M.; Kouda, Y.; Sato, K.; Minakawa, N.; Matsuda, A
  • Journal Title: Chem. Commun Volume: 48 Pages: 8700-8702
  • DOI: doi:10.1039/C1CC12980E
  • Peer Reviewed
• [Presentation] 5-(5,6-dimethoxybenzothiazol-2-yl)-2'-deoxyuridineを用いた蛍光ラベル化DNAの酵素合成 (2013)
  • Author(s): 佐藤浩輔、佐々木彩乃、松田彰
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 20130328-20130330
• [Presentation] ナフチリジンリボヌクレオチド5'-トリリン酸体の合成とT7RNAポリメラーゼによる取り込み反応の検討 (2013)
  • Author(s): 野村勇作、柏木怜、佐藤浩輔、松田彰
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 20130328-20130330
• [Presentation] 修飾ヌクレオシドの新規機能性分子への展開 (2012)
  • Author(s): 佐藤浩輔
  • Organizer: 日本薬学会北海道支部例会第139例会
  • Place of Presentation: 北海道大学学術交流会館(札幌))
  • Year and Date: 20121208-20121208
• [Presentation] ナフチリジン-イミダゾピリドピリミジン塩基対を用いた選択的転写反応の検討 (2012)
  • Author(s): 野村勇作、柏木怜、佐藤浩輔、松田彰
  • Organizer: アンチセンス・遺伝子・デリバリーシンポジウム2012
  • Place of Presentation: 仙台市民会館(宮城県)
  • Year and Date: 20120924-20120926
• [Presentation] Selective detection method for5-formyl-2〓-deoxyuridine in DNA using a fluorogenic reagent XX International Roundtable on Nucleosides (2012)
  • Author(s): 佐藤浩輔、広瀬亘、松田彰
  • Organizer: Nucleotides and Nucleic Acids
  • Place of Presentation: McGill University(カナダ)
  • Year and Date: 20120805-20120809
• [Presentation] DNA 酸化損傷の選択的検出法の開発 (2012)
  • Author(s): 佐藤浩輔
  • Organizer: 日本薬学会北海道支部例会第138例会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 20120616-20120617
• [Presentation] T7 RNA polymerase による基質トリリン酸体認識機構の解明 (2012)
  • Author(s): 柏木怜、佐藤浩輔、松田彰
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-31
• [Presentation] 4本の水素結合を有するナフチリジン-イミダゾピリドピリミジン塩基対を用いた転写反応 (2012)
  • Author(s): 野村勇作、柏木怜、佐藤浩輔、松田彰
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-03-29
• [Presentation] Development of Selective Detection Method for 5-Formyl-2'-deoxyuridine in DNA using a Fluorogenic Reagent (2011)
  • Author(s): Sato, K.; Hirose, W.; Matsuda, A
  • Organizer: ICCEOCA-6/NICCEOCA-2
  • Place of Presentation: The Chinese University of Hong Kong(香港)
  • Year and Date: 2011-12-12
• [Presentation] Synthesis of 1,8-naphthyridine C-ribonucleotides and their incorporation into RNA by T7 RNA polymerase (2011)
  • Author(s): Nomura, Y.; Kashiwagi, S.; Sato, K.; Matsuda, A
  • Organizer: The 38th International Symposium on Nucleic Acids Chemistry 2011
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-11-09
• [Presentation] Groove recognition of T7 RNA polymerase using base-modified triphosphate analogs (2011)
  • Author(s): Kashiwagi, S.; Sato, K.; Matsuda, A
  • Organizer: The 38th International Symposium on Nucleic Acids Chemistry 2011
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-11-09
• [Presentation] 糖部修飾型核酸 3'-deoxyapionucleicacid (apioNA) の性質と酵素合成 (2011)
  • Author(s): 幸田康生、片岡真由美、佐藤浩輔、松田彰
  • Organizer: アンチセンス・遺伝子・デリバリーシンポジウム 2011
  • Place of Presentation: 大阪大学(吹田市)
  • Year and Date: 2011-09-01
• [Presentation] 1,8-ナフチリジンC-リボヌクレオチドの合成とT7RNAポリメラーゼによる転写反応 (2011)
  • Author(s): 野村勇作、柏木怜、佐藤浩輔、松田彰
  • Organizer: 日本薬学会北海道支部例会第136例会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2011-05-21
• [Remarks]
  • URL: http://www.pharm.hokudai.ac.jp/yakka/Matsuda_Lab/Matsuda_Lab_Home.html