2012 Fiscal Year Final Research Report
In situ measurements of mechanical forces in biomolecular interactions
| Project/Area Number |
23750183
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
FURUSAWA Hiroyuki 東京工業大学, 大学院・生命理工学研究科, 助教 (60345395)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 生体機能関連化学 / 生体分子間相互作用 / 機械的力 / 水晶発振子 / RNA シャペ ロンタンパク質 Hfq |
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| Research Abstract |
The aim of this study is development of a biosensor, which can observe biomolecular interactions, combined with a unit to investigate effects of mechanical force on biomolecules. In this study, a flow-type quartz-crystal microbalance, which can detect biomolecular interactions as a mass change, was developed as a base device combined with various units easily. In addition, kinetic analysis of RNA chaperon Hfq was performed to discus the contribution of molecular mechanics in the biomolecular interactions.
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