2013 Fiscal Year Final Research Report
Preparation and Application of polythiophene/protein conjugate
| Project/Area Number |
23750187
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Nagoya Institute of Technology |
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Principal Investigator |
MIZUNO TOSHIHSIA 名古屋工業大学, 工学(系)研究科(研究院), 助教 (90345950)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 導電性高分子 / 蛋白質 / ナノ粒子 / 蛍光プローブ |
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| Research Abstract |
Preparations of poly (3,4-ethylenedioxythiophene)/protein (PEDOT/protein) nanoparticle, consisting of the oligomeric PEDOTs and natural proteins were achieved by an oxidative polymerization of 3,4-ethylenedioxythiophene (EDOT) in the presence of partially denatured proteins in an aqueous solution containing 3 M Gdm HCl. From the dynamic light scattering (DLS) and the transmission electron microscope (TEM) observations, the hydrodynamic diameters of obtained nanoparticles were homogeneous by ~ 50 nm. We evaluated the redox potentials of PEDOT/protein nanoparticles using the cyclic voltammetric analyses and the observed redox potentials have a dependence on the choice of natural proteins. The current method to prepare the PEDOT/protein nanoparticle should be applicable to construct various kinds of the PEDOT/biomolecule nanoparticles, leading to the construction of redox-active sensor conjugates enable to use in vitro and in vivo.
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Research Products
(26 results)
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[Journal Article] Applications of Peptide Gemini Surfactants as Solubilization Surfactants for Photosystems I and II of Cyanobacteria2013
Author(s)
S. Koeda, K. Umezaki, T. Noji, A. Ikeda, K. Kawakami, M. Kondo, Y. Yamamoto, J. Shen, K. Taga, T. Dewa, M. Nango, T. Tanaka, T. Mizuno
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Journal Title
Langmuir
Volume: 29(37)
Pages: 11667–11680
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[Journal Article] Creation of fibrous nanotube of green fluorescent protein by conjugation with pH-responsive polymer, poly (2-vinylpyridine), and use of a microfluidic synthesis2013
Author(s)
N. Okiyama, E. Ota, A. Sumino, T. Noji, K. Yamamoto, J. Oku, T. Dewa, T. Tanaka, T. Mizuno
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Journal Title
Chem. Lett.
Volume: 42(5)
Pages: 495-497
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