2012 Fiscal Year Final Research Report
Development of Chemical Protein Labeling method for Spatiotemporal Modulation of Endogenous Protein Activity in Cells
| Project/Area Number |
23750188
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAOKA Yousuke 京都大学, 大学院・工学研究科, 助教 (80599762)
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| Project Period (FY) |
2011 – 2012
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| Keywords | タンパク質 / ケミカルバイオロジー / 化学修飾 / 酵素 |
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| Research Abstract |
We developed a novel method for one-step construction of caged enzymes using carbonic anhydrase I (CAI) as a model enzyme. This method is simple and based on the transient tethering of an inhibitor with moderate activity that is directed to the active site on an enzyme surface. We successfully showed that the activity of the caged CAI was almost completely suppressed by Ligand-directed acyl imidazole (LDAI) based labeling and fully recovered by photoirradiation in the crude conditions as well as in test tube settings.
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