2013 Fiscal Year Final Research Report
Studies on interprotein electron transfer using artificial protein complexes
| Project/Area Number |
23750190
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemistry related to living body
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| Research Institution | Osaka University |
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Principal Investigator |
NOJIRI Masaki 大阪大学, 理学(系)研究科(研究院), 講師 (20333346)
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| Project Period (FY) |
2011 – 2013
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| Keywords | 電子移動反応 / 電子伝達蛋白質 / 蛋白質複合体 / 過渡的複合体 / X線結晶構造解析 / ブルー銅蛋白質 / シトクロムc / 亜硝酸還元酵素 |
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| Research Abstract |
Generally, the redox-partner proteins form a transient complex during the inter-protein electron transfer reaction. It has been known that there are some modulation mechanisms for effective interportein ET due to the conformational changes in the complex structure. However, most of the details for modulation remain unknown, because it is quite difficult to determine the complex structure due to the instability. So, we planed to analyze the structures and biophysical properties for the complexes constructed artificially and the naturally fused proteins. Based on their data, we discuss and propose the unprecedented mechanism for interprotein ET reaction.
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[Journal Article] Structural and functional characterization of the Geobacillus copper nitrite reductase : involvement of the unique N-terminal region in the interprotein electron transfer with its redox partner2014
Author(s)
Fukuda, Y., Koteishi, H., Yoneda, R., Tamada, T., Takami, H., Inoue, T., Nojiri, M.
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Journal Title
Biochim. Biophys. Acta
Volume: 1837
Pages: 396-405
Peer Reviewed
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[Journal Article] Structural insights into the function of a thermostable copper-containing nitrite reductase2014
Author(s)
Fukuda, Y., Tse, K. M., Lintuluoto, M., Fukunishi, Y., Mizohata, E., Matsumura, H., Takami, H., Nojiri, M., Inoue, T.
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Journal Title
J. Biochem.
Volume: 155
Pages: 123-135
Peer Reviewed
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[Journal Article] The structure of the cytochrome p450cam-putidaredoxin complex determined by paramagnetic NMR spectroscopy and crystallography2013
Author(s)
Hiruma, Y., Hass, M. A., Kikui, Y., Liu, W. M., Ölmez, B., Skinner, S. P., Blok, A., Kloosterman, A., Koteishi, H., Löhr, F., Schwalbe, H., Nojiri, M., Ubbink, M.
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Journal Title
J. Mol. Biol.
Volume: 425
Pages: 4353-4365
Peer Reviewed
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[Journal Article] Structural and mechanistic insights into the electron-flow through protein for cytochrome c-tethering copper nitrite reductase2013
Author(s)
Tsuda, A., Ishikawa, R., Koteishi, H., Tange, K., Fukuda, Y., Kobayashi, K., Inoue, T., Nojiri, M.
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Journal Title
J. Biochem.
Volume: 154
Pages: 51-60
Peer Reviewed
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[Journal Article] Cloning, expression, purification, crystallization and preliminary X-ray crystallographic study of GK0767, the copper-containing nitrite reductase from Geobacillus kaustophilus2011
Author(s)
Fukuda, Y., Tamada, T., Takami, H., Suzuki, S., Inoue, T., Nojiri, M.
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Journal Title
Acta Cryst
Volume: F67
Pages: 692-695
Peer Reviewed
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