Effects of physical stresses generated by underwater pulseddischarges on transcriptional activation of retrotransposon of Porphyra yezoensis

2012 Fiscal Year Final Research Report

• Project/Area Number: 23760265
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Power engineering/Power conversion/Electric machinery
• Research Institution: Kumamoto University
• Principal Investigator: WANG Douyan 熊本大学, 大学院・先導機構, 准教授 (30508651)
• Project Period (FY): 2011 – 2012
• Keywords: 電気エネルギー工学 / バイオエレクトリクス

Research Abstract

Underwater pulsed discharge is an active stress for the transcriptional activation ofretrotransposon genes of Porphyra yezoensis. The physical phenomena related to theunderwater pulsed discharges: chemically active species, high pulsed electric fields,large current and ultraviolet rays, act as a synergistic method for the transcriptionalactivation of the genes. This research project deduced that the synergistic effect isstronger than each single physical phenomenon in order to obtain a higher level of geneexpression.

Research Products

• [Journal Article] Effectsof pulsed electric field and pulsedcurrent on transcriptional activation ofretrotransposon of Porphyra yezoensis (2012)
  • Author(s): K. Hirayama、D. Wang、T. Namihira、H.Takano、S. Takio and H. Akiyama
  • Journal Title: International Journal of PlasmaEnvironmental Science & Technology Volume: Vol.6、No.3 Pages: 272-277
  • Peer Reviewed
• [Journal Article] Akiyama、Concreterecycling by pulsed power discharge insideconcrete (2012)
  • Author(s): S. Inoue、S.Iizasa、D. Wang、T. Namihira、M. Shigeishi、M. Ohtsu、H
  • Journal Title: International Journal of PlasmaEnvironmental Science & Technology Volume: Vol.6、No.2 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Protein Transduction into EukaryoticCells Using Non-thermal Plasma (2012)
  • Author(s): N. Takamura、D. Wang、D. Seki、T. Namihira、Ken-ichi Yano、H. Saitoh and H. Akiyama
  • Journal Title: International Journal of PlasmaEnvironmental Science & Technology Volume: Vol.6、No.1 Pages: 59-62
  • Peer Reviewed
• [Journal Article] Propagation of Streamer Heads During a5-ns Pulsed Discharge (2011)
  • Author(s): D. Wang、T. Namihira and H. Akiyama
  • Journal Title: IEEE Transactions on Plasma Science of Special Issue on Images in Plasma Science Volume: Vol.39、No.11 Pages: 2268-2269
  • Peer Reviewed
• [Journal Article] Discharge Appearances of 2- and5-ns Pulsed Power (2011)
  • Author(s): T. Matsumoto、D. Wang、T. Namihira andH. Akiyama
  • Journal Title: IEEE Transactions on Plasma Science of Special Issue on Images in Plasma Science Volume: Vol.39、No.11 Pages: 2262-2263
  • DOI: DOI:10.1109/TPS.2011.2160878
  • Peer Reviewed
• [Journal Article] StreamerPropagation of Nanosecond Pulse DischargeWith Various Rise Times (2011)
  • Author(s): I. Yagi、S. Okada、T. Matsumoto、D. Wang、T. Namihira 、K. Takaki
  • Journal Title: IEEE Transactionson Plasma Science of Special Issue onImages in Plasma Science Volume: Vol.39、No.11 Pages: 2232-2233
  • DOI: DOI:10.1109/TPS.2011.215438
  • Peer Reviewed
• [Journal Article] Process performances of 2nspulsed discharge plasma (2011)
  • Author(s): T. Matsumoto、D. Wang、T. Namihira、H.Akiyama
  • Journal Title: Japanese Journalof Applied Physics Volume: Vol.50、No.8
  • DOI: DOI:10.1143/JJAP.50.08JF14
  • Peer Reviewed
• [Journal Article] Spatiotemporal distribution ofnitrogen rotational temperature duringpulsed discharge in air (2011)
  • Author(s): S. Okada、D. Wang、T. Namihira、S. Katsuki、H. Akiyama
  • Journal Title: Japanese Journalof Applied Physics Volume: Vol.50、No.8
  • DOI: DOI:10.1143/JJAP.50.08JB06
  • Peer Reviewed
• [Journal Article] Energy TransferEfficiency of Nano-Seconds Pulsed PowerGenerator for Nonthermal PlasmaProcessing Technique (2011)
  • Author(s): D. Wang、M. Matsuda、T. Matsumoto、T.Namihira 、H. Akiyama
  • Journal Title: IEEE Transactions onDielectrics and Electrical Insulation Volume: Vol.18、No.4 Pages: 1091-1096
  • DOI: DOI:10.1109/TDEI.2011.5976100
  • Peer Reviewed
• [Presentation] Performance of waste watertreatment system based on nano-secondspulsed discharge (2012)
  • Author(s): Y. Shimasaki, D. Wang, T. Namihira, H.Akiyama
  • Organizer: 18th InternationalConference on Advanced OxidationTechnologies for Treatment of Water
  • Place of Presentation: Crowne PlazaRiverfront, Jacksonville, Florida, USA
  • Year and Date: 20121111-15
• [Presentation] Effect of rise timeof applied pulsed voltage on ozonegeneration characteristics (2012)
  • Author(s): T. Hirota, Y. Maruta, D. Wang, T.Namihira, H. Akiyama
  • Organizer: International Conference on AdvancedOxidation Technologies for Treatment ofWater, Air and Soil
  • Place of Presentation: CrownePlaza Riverfront, Jacksonville, Florida,USA
  • Year and Date: 20121111-15
• [Presentation] Radial distribution of ozonein a coaxial reactor of nano-secondspulsed discharge based ozonizer (2012)
  • Author(s): S. Naka, R. Tsuruga, D. Wang, T. Namihira,H. Akiyama
  • Organizer: 8thInternational Conference on AdvancedOxidation Technologies for Treatment ofWater, Air and Soil
  • Place of Presentation: CrownePlaza Riverfront, Jacksonville, Florida,USA.
  • Year and Date: 20121111-15
• [Presentation] Effect of electrodetemperature on ozone production usingnano-seconds pulsed discharge (2012)
  • Author(s): Y. Araki, K. Yamawaki, D. Wang, T.Namihira, H. Akiyama
  • Organizer: 18thInternational Conference on AdvancedOxidation Technologies for Treatment ofWater, Air and Soil
  • Place of Presentation: CrownePlaza Riverfront, Jacksonville, Florida,USA
  • Year and Date: 20121111-15
• [Presentation] Process performance of nano-secondspulsed discharge (2012)
  • Author(s): T. Namihira, D. Wang, H. Akiyama
  • Organizer: 18th InternationalConference on Advanced OxidationTechnologies for Treatment of Water, Airand Soil
  • Place of Presentation: Crowne PlazaRiverfront, Jacksonvill, Florida, USA
  • Year and Date: 20121111-15
• [Presentation] Generation and characteristicsurvey of atmospheric-pressure dry, vapor,mist plasma jet using high-frequency high-voltage power supply (2012)
  • Author(s): Crowne PlazaRiverfront, Jacksonvill, Florida, USA
  • Organizer: 5th GaseousElectronics Conference
  • Place of Presentation: AT&T Conference Center, Austin, USA
  • Year and Date: 20121022-26
• [Presentation] ナノ秒パルス放電方式オゾナイザにおける電極内ガス温度及び電極冷却効果の検証 (2012)
  • Author(s): 柳田宗正, 澁田哲, 王斗艷, 浪平隆男,秋山秀典
  • Organizer: パルスパワー・放電同研究会
  • Place of Presentation: 佐賀大学(佐賀)
  • Year and Date: 20121022-23
• [Presentation] ナノ秒パルス放電を用いたオゾン生成における電極温度の影響 (2012)
  • Author(s): 平下友貴, 荒木良孝, 王斗艷, 浪平隆男,秋山秀典
  • Organizer: パルスパワー・放電同研究会
  • Place of Presentation: 佐賀大学(佐賀)
  • Year and Date: 20121022-23
• [Presentation] Effects of reactorcooling for nano-seconds pulsed dischargebased ozonizer (2012)
  • Author(s): S. Shibuta, K.Yamawaki. D. Wang, T.Namihira, H. Akiyama
  • Organizer: 4th Euro-Asian PulsedPower Conference and 19th InternationalConference on High-Power Particle Beams
  • Place of Presentation: Karlsruhe Institute ofTechnology, Karlsruhe, German.
  • Year and Date: 20120930-1004
• [Presentation] Effect ofatmospheric-pressure plasma on cellculture media and cell death (2012)
  • Author(s): N. Takamura, D. Wang, T. Namihira, K.Yano, H. Saitoh, H. Akiyama
  • Organizer: 4thEuro-Asian Pulsed Power Conference and19th International Conference onHigh-Power Particle Beams
  • Place of Presentation: Karlsruhe Institute ofTechnology, Karlsruhe, German
  • Year and Date: 20120930-1004
• [Presentation] Potential of laser breakdownplasma on new chemical reaction field (2012)
  • Author(s): R. Tsuruga, S. Naka, D. Wang, T. Namihira,H. Akiyama
  • Organizer: 4th Euro-Asian Pulsed Power Conference and19th International Conference onHigh-Power Particle Beams
  • Place of Presentation: Karlsruhe Institute ofTechnology, Karlsruhe, German.
  • Year and Date: 20120930-1004
• [Presentation] Development ofozonizer using nano-seconds pulseddischarge (2012)
  • Author(s): K. Yamawaki, S.Shibuta, D. Wang, T.Namihira and H. Akiyama
  • Organizer: 4th Euro-Asian Pulsed PowerConference and 19th InternationalConference on High-Power Particle Beams
  • Place of Presentation: Karlsruhe Institute ofTechnology, Karlsruhe, German
  • Year and Date: 20120930-1004
• [Presentation] ナノ秒パルス放電によるオゾン生成におけるリアクタ冷却効果 (2012)
  • Author(s): 澁田哲, 王斗艷, 浪平隆男, 秋山秀典
  • Organizer: 平成 24 年度(第65回)電気関係学会九州支部連合大会
  • Place of Presentation: 長崎大学(長崎)
  • Year and Date: 20120924-25
• [Presentation] New chemical reactionfield produced by laser breakdown plasmain solution (2012)
  • Author(s): R. Tsuruga, S. Naka, D. Wang, T. Namihiraand H. Akiyama
  • Organizer: 9th InternationalBioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto, Kumamoto, Japan.
  • Year and Date: 20120905-08
• [Presentation] Oxidation and de-oxidationperformances of nano-seconds pulseddischarge (2012)
  • Author(s): Y. Shimasaki, D. Wang, T. Namihira andH. Akiyama
  • Organizer: 9th InternationalBioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto, Kumamoto, Japan.
  • Year and Date: 20120905-08
• [Presentation] Energy transfer efficiency onnano-seconds pulsed discharge system (2012)
  • Author(s): Y. Araki, D. Wang, T. Namihira, and H.Akiyama
  • Organizer: 9th International Bioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto,Kumamoto, Japan
  • Year and Date: 20120905-08
• [Presentation] Effect of risetime of applied pulsed voltage on streamerhead property (2012)
  • Author(s): T. Hirota, Y. Maruta, D. Wang, T.Namihira and H. Akiyama
  • Organizer: 9th InternationalBioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto, Kumamoto, Japan
  • Year and Date: 20120905-08
• [Presentation] Performances ofnanoseconds pulsed generator (2012)
  • Author(s): K. Yamawaki, S. Shibuta, D. Wang, T.Namihira and H. Akiyama
  • Organizer: International Bioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto,Kumamoto, Japan
  • Year and Date: 20120905-08
• [Presentation] Gas temperature trend duringozone generation using nano-secondspulsed discharge (2012)
  • Author(s): S. Shibuta, D. Wang, T. Namihira, H.Akiyama
  • Organizer: 9th InternationalBioelectrics Symposium
  • Place of Presentation: KKR Hotel Kumamoto,Kumamoto, Japan
  • Year and Date: 20120905-08
• [Presentation] Growth control ofleaf vegetables using pulsed electricfield (2012)
  • Author(s): D. Wang, T. Sonoda, T. Namihira, H.Takano, H. Akiyama
  • Organizer: 9th International BioelectricsSymposium
  • Place of Presentation: KRHotel Kumamoto, Kumamoto, Japan.
  • Year and Date: 20120905-08
• [Presentation] Proteinand antibody transduction into human cellsusing non-thermal plasma (2012)
  • Author(s): N. Takamura, D. Wang, T. Namihira , K.Yano, H. Saitoh, and H. Akiyama
  • Organizer: 9thInternational Bioelectrics Symposium
  • Place of Presentation: KKR HotelKumamoto, Kumamoto, Japan
  • Year and Date: 20120905-08
• [Presentation] 2ns パルス放電プラズマの諸特性 (2012)
  • Author(s): 山脇和敏, 王斗艶, 浪平隆男, 秋山秀典
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] ナノ秒パルス放電の酸化・還元能 (2012)
  • Author(s): 島崎祐輔, 王斗艶, 浪平隆男, 秋山秀典
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] ナノ秒パルス放電システムのエネルギー転送効率 (2012)
  • Author(s): 荒木良孝, 王斗艶, 浪平隆男, 秋山秀典
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] ns パルスパワーによる分布定数様放電の観測 (2012)
  • Author(s): 丸田康博, 廣田智行, 王斗艶, 浪平隆丸田康博, 廣田智行, 王斗艶, 浪平隆
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] ストリーマヘッド諸特性の印加電圧依存性 (2012)
  • Author(s): 廣田智行, 丸田康博, 王斗艶, 浪平隆男, 秋山秀典
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] ナノ秒パルス放電の紹介 (2012)
  • Author(s): 浪平隆男, 王斗艶, 秋山秀典
  • Organizer: プラズマ・パルスパワー合同研究会
  • Place of Presentation: 岩手大学(岩手)
  • Year and Date: 20120808-10
• [Presentation] パルス電界によるレタスの成育制御 (2012)
  • Author(s): 王斗艶, 後藤貴弘, 吉田尭史, 浪平隆男, 秋山秀典, 藤原俊之, 佐藤大吾
  • Organizer: パルスパワー研究会
  • Place of Presentation: 熊本大学(熊本)
  • Year and Date: 20120308-09
• [Presentation] オゾン生成に対するパルス電圧立ち上がり時間の影響 (2012)
  • Author(s): 廣田智行, 丸田康博, 王斗艶, 浪平隆男,秋山秀典
  • Organizer: 放電研究会
  • Place of Presentation: 兵庫県立大学(兵庫)
  • Year and Date: 2012-12-15
• [Presentation] ガス種に対するストリーマヘッドの挙動特性 (2012)
  • Author(s): 丸田康博, 廣田智行, 王斗艶, 浪平隆男,秋山秀典
  • Organizer: 放電研究会
  • Place of Presentation: 兵庫県立大学(兵庫)
  • Year and Date: 2012-12-15
• [Presentation] Protein Transduction intoEukaryotic Cells Using Non-thermalPlasma (2011)
  • Author(s): N. Takamura, D. Wang, D. Seki, T.Namihira, Ken-ichi Yano, H. Saitoh and H.Akiyama
  • Organizer: 2nd International Symposium onPlasma Environmental, Atmosphericelectricity, Space charge, Electricaldischarge and Static electricity,Applications and Prevention Science andTechnology
  • Place of Presentation: IRD Auditorium,Noumea, New Caledonia.
  • Year and Date: 20111114-18
• [Presentation] Axialpropagation of nano-seconds pulseddischarge in coaxial reactor (2011)
  • Author(s): T. Matsumoto, S. Okada, T. Hirota. D.Wang, T. Namihira, H. Akiyama
  • Organizer: 2ndInternational Symposium on PlasmaEnvironmental, Atmospheric electricity,Space charge, Electrical discharge andStatic electricity, Applications andPrevention Science and Technology
  • Place of Presentation: IRD Auditorium, Noumea,New Caledonia.
  • Year and Date: 20111114-18
• [Presentation] Influence of reactorconfiguration on ozone generation bynano-seconds pulsed discharges (2011)
  • Author(s): T. Matsumoto, D. Wang, T. Namihira, H.Akiyama
  • Organizer: 2ndInternational Symposium on PlasmaEnvironmental, Atmospheric electricity,Space charge, Electrical discharge andStatic electricity, Applications andPrevention Science and Technology
  • Place of Presentation: IRD Auditorium, Noumea,New Caledonia
  • Year and Date: 20111114-18
• [Presentation] Process performances of 2 nspulsed discharge plasma (2011)
  • Author(s): T. Namihira, T. Matsumoto, D. Wang, H.Akiyama
  • Organizer: 2ndInternational Symposium on PlasmaEnvironmental, Atmospheric electricity,Space charge, Electrical discharge andStatic electricity, Applications andPrevention Science and Technology
  • Place of Presentation: IRD Auditorium, Noumea,New Caledonia
  • Year and Date: 20111114-18
• [Presentation] Phenoltreatment by laser produced plasma inpressurized solution (2011)
  • Author(s): R. Tsuruga, K. Kohtake, S. Naka, D.Wang, T. Namihira, H. Akiyama
  • Organizer: 17th InternationalConference on Advanced OxidationTechnologies for Treatment of Water, Airand Soil
  • Place of Presentation: Town & CountryResort, San Diego, California, USA.
  • Year and Date: 20111107-10
• [Presentation] Degradation of phenol bynano-seconds pulsed discharge on solutionsurface (2011)
  • Author(s): Y. Shimasaki, D. Wang, T. Namihira, H.Akiyama
  • Organizer: 17th International Conferenceon Advanced Oxidation Technologies forTreatment of Water, Air and Soil
  • Place of Presentation: Town & Country Resort, SanDiego, California, USA
  • Year and Date: 20111107-10
• [Presentation] Environmental application ofnano-seconds pulsed discharge system (2011)
  • Author(s): T. Namihira, D. Wang, H. Akiyama
  • Organizer: 17th International Conference on AdvancedOxidation Technologies for Treatment ofWater, Air and Soil
  • Place of Presentation: Town& Country Resort, San Diego, California,USA
  • Year and Date: 20111107-10
• [Presentation] ns パルス放電によるオゾン生成特性 (2011)
  • Author(s): 澁田哲, 山脇和敏, 松本宇生, 王斗艶,浪平隆男, 秋山秀典
  • Organizer: パルスパワー・放電合同研究会
  • Place of Presentation: 千葉工業大学(千葉)
  • Year and Date: 20111021-22
• [Presentation] 非熱平衡プラズマの形成効率に優れた ns パルス放電システム (2011)
  • Author(s): 山脇和敏, 澁田哲, 松本宇生, 王斗艶,浪平隆男, 秋山秀典
  • Organizer: パルスパワ・放電合同研究会
  • Place of Presentation: 千葉工業大学(千葉)
  • Year and Date: 20111021-22
• [Presentation] パルスパワーによるウミグモの電気的駆除 (2011)
  • Author(s): 王斗艶, 平山勝将, 高村紀充, 後藤貴弘, 浪平隆男, 秋山秀典
  • Organizer: 平成 23 年電気学会基礎材料共通部門大会
  • Place of Presentation: 東京工業大学(東京)
  • Year and Date: 20110921-22
• [Presentation] 大気圧プラズマジェットによる細胞内へのタンパク質導入 (2011)
  • Author(s): 王斗艶, 高村紀充, 浪平隆男, 秋山秀典
  • Organizer: 平成 23 年電気学会基礎材料共通部門大会
  • Place of Presentation: 東京工業大学(東京)
  • Year and Date: 20110921-22
• [Presentation] 全固体ナノ秒パルス電源を用いたストリーマ進展速度と発光分光計測 (2011)
  • Author(s): 八木一平, 高木浩一, 岡田翔, 松本宇生, 王斗艶, 浪平隆男, 小野亮, 小田哲治
  • Organizer: 第35回静電気学会全国大会
  • Place of Presentation: 東京理科大学(東京)
  • Year and Date: 20110912-13
• [Presentation] Studies ofElectrical Killing on Nymphonella tapetis Using Pulsed Power Technology (2011)
  • Author(s): D. Wang, K. Hirayama, N. Takamura, T.Goto, T. Namihira, H. Akiyama
  • Organizer: Proceeding of the XXX InternationalConference on Phenomena in Ionized Gases(ICPIG)
  • Place of Presentation: The Univ. ofYork, Belfast, Northern Ireland, UK.
  • Year and Date: 20110828-0902
• [Presentation] Ozone generationusing positive- and negativenano-seconds pulsed discharges (2011)
  • Author(s): N. Takamura, T. Matsumoto, D. Wang, T.Namihira, H. Akiyama
  • Organizer: 18thIEEE International Pulsed PowerConference
  • Place of Presentation: HyattRegency-McCormick Place, Chicago,Illinois, USA.
  • Year and Date: 20110619-23
• [Presentation] Gas temperature measurementsof nano-seconds pulsed discharge basedozonizer (2011)
  • Author(s): T. Matsumoto, D. Wang, T. Namihira andH. Akiyama
  • Organizer: 18th IEEE International PulsedPower Conference
  • Place of Presentation: yattRegency-McCormick Place, Chicago,Illinois, USA
  • Year and Date: 20110619-23
• [Presentation] Axialpropagation of nano-seconds pulseddischarge in coaxial reactor (2011)
  • Author(s): T. Hirota, S. Okada, D. Wang, T.Namihira and H. Akiyama
  • Organizer: 18th IEEEInternational Pulsed Power Conference
  • Place of Presentation: Hyatt Regency-McCormickPlace, Chicago, Illinois, USA
  • Year and Date: 20110619-23
• [Presentation] Toluene decomposition usingnano-seconds pulsed discharge (2011)
  • Author(s): Y. Araki, D. Wang, T. Namihira, H.Akiyama
  • Organizer: 18th IEEEInternational Pulsed Power Conference
  • Place of Presentation: Hyatt Regency-McCormickPlace, Chicago, Illinois, USA.
  • Year and Date: 20110619-23
• [Book] Air Pollution - AComprehensive Perspective (2012)
  • Author(s): T. Matsumoto, D. Wang, T. Namihira, H.Akiyama
  • Total Pages: 215-234
  • Publisher: InTech
• [Patent(Industrial Property Rights)] 物質導入用液体培地及び細胞内への物質導入方法 (2011)
  • Inventor(s): 王斗艶、関大亮、浪平隆男、齊藤壽仁、秋山秀典権利者:熊本
  • Industrial Property Rights Holder: 熊本大学
  • Industrial Property Number: 13/699,564
  • Filing Date: 2011-05-25