2014 Fiscal Year Final Research Report
Kinetic analysis of cellulosic biomass degradation based on an electrochemical measurement combined with calorimetry
| Project/Area Number |
23760746
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Research Collaborator |
CRUYS-BAGGER Nicolaj ロスキレ大学, 博士研究員
BORCH Kim ノボザイム, 研究員
WESTH Peter ロスキレ大学, 教授
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Keywords | バイオマス / バイオセンサー / カロリメトリー |
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| Outline of Final Research Achievements |
The kinetics of the enzymatic hydrolysis of cellulosic biomass was studied by real-time monitoring with amperometic biosensor and calorimetry. The response time of the biosensor was improved to follow a rapid change in reaction rates at a pre-steady state of cellobiohydrolase reaction. By the kinetic analysis, it was concluded that the rapid decrease in the rate of cellobiohydrolase reaction at a pre-steady state was derived from the slow dissociation of the enzyme from a cellulose chain.
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| Free Research Field |
電気分析化学
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