2013 Fiscal Year Final Research Report
Development of high-throughput human protein expression and purification system in silkworms
| Project/Area Number |
23760747
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Shizuoka University |
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Principal Investigator |
TATSUYA Kato 静岡大学, (連合)農学研究科(研究院), 准教授 (00397366)
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| Project Period (FY) |
2011 – 2013
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| Keywords | カイコ / ハイスループット / タンパク質発現 |
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| Research Abstract |
Multi-expression and purification of recombinant proteins in BmNPV bacmid-silkworm expression system were constructed. Many kinds of recombinant BmNPV bacmid DNA were prepared in a 96 well plate and recombinant proteins were also purified in a 96 well plate packing a purification gel. The further improvement of protein extraction and purification step leads to the establishment of a more practical silkworm system.
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