Construction of assay method for detection of anti-GPCR antibody

2012 Fiscal Year Final Research Report

• Project/Area Number: 23760748
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Nagoya University
• Principal Investigator: AKIYAMA Shinichi 名古屋大学, 医学系研究科, 特任講師 (20500010)
• Project Period (FY): 2011 – 2012
• Keywords: ゼブラフィッシュ / 抗体測定 / ネイティブフォーム抗原 / 膜タンパク質

Research Abstract

Using zebrafish, Danio rerio, embryo which is known for next generation recombinant expression host, a recombinant human phospholipase A2 receptor (hPLA2R) protein, a kind of membrane associate protein, with native-form conformation was successfully generated. Moreover, we demonstrated that anti-hPLA2R IgG which recognize aepitope structure from patient with membranous nephropathy were successfully measured with this recombinant hPLA2R protein as antigen.

Research Products

• [Journal Article] Functional and heterologous expression of human protein O-linked mannose β -1,2-N- acetylglucosaminyltransferase 1 in zebrafish (2012)
  • Author(s): E. Avsar-Ban, H. Ishikawa, S. Akiyama, H. Manya, T. Endo, Y. Tamaru
  • Journal Title: J. Biosci. Bioengin Volume: 114(2) Pages: 237-239
  • Peer Reviewed
• [Journal Article] Tissue-restricted expression of Nrf2 and its target genes in zebrafish with gene-specific variations in the induction profiles (2011)
  • Author(s): H. Nakajima, Y. Nakajima-Takagi, T. Tsujita, S. Akiyama, T. Wakasa, K. Mukaigasa, H. Kaneko, Y. Tamaru, M. Yamamoto, M. Kobayashi
  • Journal Title: PLoS ONE Volume: 6(10) Pages: e26884
  • Peer Reviewed
• [Presentation] Prevalence of autoantibody against PLA2R1 in patients with membranous nephropathy in Japan (2012)
  • Author(s): S. Akiyama, T. Ozaki, S. Maruyama, H. Yokoyama, G. Lambeau, D. Salant, S. Matsuo, E. Imai
  • Organizer: American Society of Nephrology 2012
  • Place of Presentation: アメリカ合衆国 San diego
  • Year and Date: 2012-11-03
• [Presentation] Development of ELISA for detection of anti-phospholipase A2 receptor autoantibodies as a biomarker of idiopathic membranous nephropathy (2012)
  • Author(s): S Akiyama, S. Maruyama, S. Matsuo, E. Imai
  • Organizer: 55thAnnual meeting of the Japanese Society of Nephrology
  • Place of Presentation: Yokohama
  • Year and Date: 2012-06-01
• [Presentation] Development of ELISA for detection of anti-phospholipase A2 receptor autoantibodies as a biomarker of idiopathic membranous nephropathy (2012)
  • Author(s): S. Akiyama, S. Maruyama, S. Matsuo, E. Imai
  • Organizer: CKD Frontier
  • Place of Presentation: Nagoya
  • Year and Date: 2012-02-18
• [Presentation] 魚類発現系による活性型組換えヒト POMGnT1 タンパク質の生産 (2011)
  • Author(s): 秋山真一、アヴシャル坂恵利子、石川文啓、田守彩子、萬谷博、遠藤玉夫、田丸浩
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-27
• [Book] 薬ジャーナル 2012 年 11 月号(Vol.48 No.11)、腎疾患診療の最前線 3.膜性腎症の抗 PLA2R 抗体測定の現状と展望 (2012)
  • Author(s): 秋山真一、今井圓裕
  • Total Pages: 71(2565)-76(2570)
  • Publisher: 医薬ジャーナル社
• [Book] 日本生物工学会誌 (2012)
  • Author(s): 秋山真一
  • Total Pages: 199
• [Book] 食のバイオ計測の最前線-機能解析と安全・安心の計測を目指して- 7 バイオ計測への魚類バイオテクノロジーの応用 (2011)
  • Author(s): 秋山真一、田丸浩
  • Publisher: CMC 出版