2013 Fiscal Year Final Research Report
Roles of the FACT for heterochromatic silencing in fission yeast
| Project/Area Number |
23770001
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAHATA Shinya 北海道大学, 理学(系)研究科(研究院), 助教 (50381588)
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| Project Period (FY) |
2011 – 2013
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| Keywords | クロマチン / ヘテロクロマチン / ヒストンシャペロン / HP1 / FACT / Spt16 / Pob3 / CK2 |
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| Research Abstract |
We performed the comprehensive histone chaperone gene disruption and found that pob3 gene disrupted fission yeast shows most serious silencing defects at the peri-centromeric heterochromatin. We also identified that Swi6 recruits FACT onto the heterochromatin independetly of RNA polymerase II transcription. Our microarray analysis to compare pob3 knockout strain and swi6 knockout strain revealed that the expression patterns of ncRNA and mRNA were quite similar in both strain as if they regulate gene expression in the same pathway. In pob3 knockout strain, we found that impaired histone H2A/H2B reorganization triggers heterochromatic silencing defect at peri-centromeric region. In additon, novel histone H3K9me regions were identified by ChIP-sequencing. Mutations in the heterochromatin/euchromatin boundary factor results more peaks at these regions, suggesting the biological significance of H3K9 methylation for living cells.
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