2012 Fiscal Year Final Research Report
Mechanism of structural maintenance of mammalian rDNA cluster
| Project/Area Number |
23770009
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
AKAMATSU Yufuko 国立遺伝学研究所, 細胞遺伝研究系, 助教 (50381661)
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| Project Period (FY) |
2011 – 2012
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| Keywords | rDNA / DNA複製 / 複製フォーク進行阻害 / ゲノム再編成 |
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| Research Abstract |
To understand molecular mechanism maintaining mammalian rDNA cluster, DNA replication fork barrier (RFB) at 3' end of rRNA coding region was investigated. Using two -dimensional gel electrophoresis method, DNA replication fork movement was visualized. In mammalian cells, multiple RFB signals were detected and a part of them, as previously reported, were TTF1 dependent to block movement of replication fork. The other RFB, however, did not involve TTF-1, rather previously unknown mechanism functions. These results imply that collision between replication fork and rRNA transcription is prevented through multiple pathways in mammalian rDNA cluster.
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