A new application of compound specific nitrogen isotope ratios analysis of amino acids on feeding ecology study of wildlife

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Ecology/Environment
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: NAKASHITA Rumiko 独立行政法人森林総合研究所, 野生動物研究領域, 主任研究員 (00457839)
• Project Period (FY): 2011 – 2013
• Keywords: 野生動物 / 食性 / 安定同位体比 / アミノ酸

Research Abstract

I analyzed the compound specific nitrogen isotope ratios (d15NAA) of amino acids to reconstruct feeding habits of captive Asiatic black bears (food known) and free-ranging black bears and brown bears (food unknown). The results determined by d15Nbulk as a conventional method and by d15NAA method strongly suggested that the d15NAA method revealed more precise feeding habit of animals than the d15Nbulk method. The d15NAA method is a reliable and useful tool for reconstructing feeding habit of wildlife.

Research Products

• [Journal Article] 長野県塩尻市における閉鎖牛舎での捕獲ツキノワグマの家畜飼料依存度 (2014)
  • Author(s): 中下留美子,林秀剛,岸元良輔,鈴木彌生子,瀧井暁子,泉山茂之
  • Journal Title: 信州大学農学部AFC報告 Volume: 12 Pages: 85-90
  • Peer Reviewed
• [Journal Article] 捕まったツキノワグマは何を食べていた?-ツキノワグマの体毛に記録された食性履歴を生元素の同位体比でよみとる- (2013)
  • Author(s): 中下留美子
  • Journal Title: 菌蕈 Volume: 59 Pages: 9-15
• [Journal Article] 森の研究わたしのテーマ第32回「体毛から読み解くツキノワグマの食性履歴」 (2013)
  • Author(s): 中下留美子
  • Journal Title: グリーンパワー Volume: 417 Pages: 12
• [Journal Article] Ecological application of compound- specific stable nitrogen isotope analysis of amino acids?A case study for captive and wild bears (2011)
  • Author(s): R. Nakashita, Y. Suzuki, F. Akamatsu, Y. I. Naito, M. Hashimoto, T. Tsubota
  • Journal Title: Researches in Organic Geochemistry Volume: 27 Pages: 73-79
  • Peer Reviewed
• [Presentation] Feeding habits of brown bears (Ursus arctos) of the Southern Kurils islands revealed by multi-stable isotope analysis (2013)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: International Association for Ecology
  • Place of Presentation: London, UK.
  • Year and Date: 2013-08-22
• [Presentation] 生元素安定同位体比解析からみた国後島および択捉島のヒグマの食生態 (2013)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: Japan Geoscience Union Meeting 2013
  • Place of Presentation: 千葉県幕張メッセ.
  • Year and Date: 2013-05-23
• [Presentation] Estimation of the trophic position based on stable nitrogen isotopic composition of amino acids?Evaluation of hibernation effect for Asiatic black bears (2012)
  • Author(s): R. Nakashita, Y. Suzuki, M. Shimozuru, Y. Chikaraishi, N. Ohkouchi, T. Tsubota
  • Organizer: International Conference on Bear Research and Management
  • Place of Presentation: Deli, India
  • Year and Date: 2012-11-26
• [Presentation] Feeding ecology of brown bears of the Southern Kurils revealed by stable isotope analysis (2012)
  • Author(s): R. Nakashita, Y. Suzuki, K. Kobayashi, T. Ito, H. Nakamura, Y. Masuda, K. Kawai, A. Loguntsev, N. Ohtaishi, Y.Sato
  • Organizer: The 8th International Conference on Applications of Stable Isotope Techniques to Ecological Studies
  • Place of Presentation: Brest, France
  • Year and Date: 2012-08-22
• [Presentation] 生元素安定同位体比分析を用いて国後島白いヒグマの生態を探る (2012)
  • Author(s): 中下留美子,小林喬子,鈴木彌生子,伊藤哲治,中村秀次,増田泰,河合久仁子,Andrey Loguntsev,大泰司紀之,佐藤喜和
  • Organizer: 第72回分析化学討論会
  • Place of Presentation: 鹿児島大学
  • Year and Date: 2012-05-20
• [Presentation] アミノ酸窒素安定同位体比によるツキノワグマの食性解析 (2011)
  • Author(s): 中下留美子,鈴木彌生子,佐藤美穂,坪田敏男
  • Organizer: 日本哺乳類学会2011年度大会
  • Place of Presentation: 宮崎市
  • Year and Date: 20110909-10