2013 Fiscal Year Final Research Report
How do SUMO regulate cell cycle progression in Arabidopsis?
| Project/Area Number |
23770053
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Kumamoto University (2013)
Nara Institute of Science and Technology (2012)
The Institute of Physical and Chemical Research (2011) |
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Principal Investigator |
ISHIDA Takashi 熊本大学, 自然科学研究科, 特任助教 (00462656)
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| Project Period (FY) |
2011 – 2012
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| Keywords | SUMO / 細胞周期 / シロイヌナズナ |
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| Research Abstract |
Scrupulously designed cell cycle regulation is the fundamental mechanism for development of higher organisms. SUMO-mediated posttranslational modification appeared to be involved in the developmental regulation since the fact that a mutation in SUMO E3 ligase HPY2 affect cell cycle progression have reported in Arabidopsis. However, further analyses are needed to understand the molecular mechanism. In this study, we analyzed cell cycle regulators, e.g. CDKs, AURs and RBR on the SUMO-mediated cell cycle regulation context. We established experimental conditions to express and purify these proteins. Our semi-in vitro SUMOylation assays showed that the cell cycle regulators are SUMOylated proteins. Then we identified SUMOylation sites for the substrates. Furthermore, we generated binary vectors harboring SUMO-null version of the regulators and introduced into their mutants plants. The mutant version lose their functions, suggesting that the SUMOylation in essential for their functions.
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[Journal Article] The ATM-dependent DNA damage response acts as an upstream trigger for compensation in the fas1 mutation during Arabidopsis leaf development2013
Author(s)
Hisanaga T, Ferjani A, Horiguchi G, Ishikawa N, Fujikura U, Kubo M, Demura T, Fukuda H, Ishida T, Sugimoto K, Tsukaya H
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Journal Title
Plant Physiol
Volume: Volume 162
Pages: 831-841
Peer Reviewed
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[Presentation] CLAVATAシグナル伝達に関与する新規因子の探索2013
Author(s)
Breuer C, Morohashi K, Kawamura A, Takahashi N, Ishida T, Umeda M, Grotewold E, Sugimoto K,石田喬志
Organizer
植物科学若手研究2013
Place of Presentation
臨江館(愛知県犬山市)
Year and Date
2013-12-07
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