2012 Fiscal Year Final Research Report
Study of calcitonin (CT) family in an amphioxus, Branchiostoma floridae : Coevolution among CT peptide, CT receptor, and receptor activity- modifying protein.
| Project/Area Number |
23770069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphology/Structure
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| Research Institution | Kanazawa University (2012)
Suntory Foundation for Life Sciences (2011) |
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Principal Investigator |
SEKIGUCHI Toshio 金沢大学, 環日本海域環境研究センター, 助教 (40378568)
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| Project Period (FY) |
2011 – 2012
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| Keywords | Calcitonin / Calcitonin receptor / RAMP / ナメクジウオ |
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| Research Abstract |
Calcitonin (CT) family shows high molecular and functional diversification in vertebrates. To elucidate the origin of CT family, CT peptide, CT receptor (CTR), and receptor activity-modifying protein (RAMP) were identified from an amphioxus, Branchiostoma floridae, which is close animal of vertebrates. Furthermore, analysis ofCTR and RAMP expressed in mammalian cell line demonstrated that co-expression of CTR and RAMP is required for ligand activity of CT peptide, and that RAMP is necessary for cell-surface translocation of CTR. These results let us conclusion that CT peptide, CTR, and RAMP have already existed in the common ancestor between vertebrates and amphioxus.
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