Elucidation of the plant sex-determining gene using minute processing of the Y chromosome and expression profiling.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770070
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: KAZAMA Yusuke 独立行政法人理化学研究所, イオンビーム育種研究チーム, 研究員 (80442945)
• Research Collaborator: KAWANO Shigeyuki 東京大学, 大学院新領域創成科学研究科, 教授 (70161338) ; ISHII Kotaro 独立行政法人理化学研究所, 生物照射チーム, 特別研究員 (50632965) ; CHARLESWORTH Deborah School of Biological Sciences, University of Edinburgh, UK, Institute of Evolutionary Biology ; FILATOV Dimitry University of Oxford, UK, Department of Plant Science,
• Project Period (FY): 2011 – 2012
• Keywords: 重イオンビーム / 雌雄異株植物 / ヒロハノマンテマ / 突然変異

Research Abstract

Seventeen hermaphrodite mutants and ten asexual mutants that have deletions on the Y chromosome were produced by heavy-ion irradiation in the dioecious plant Silene latifolia. By performing next-generation sequencing, 1741 of Y-linked fragments were identified. The regions for sex determining genes were narrowed down by identifying commonly deleted fragment in hermaphrodite or asexual mutants, respectively. Expression-profiling method using laser microdissection was developed to determine genes expressing in the sex-specific tissues.

Research Products

• [Journal Article] Evaluation of LET-dependent effect in heavy-ion mutagenesis using rapid mutation detection system in Arabidopsis thaliana. (2013)
  • Author(s): Kazama Y, Ma L, Hirano T, Ohbu S, Shirakawa Y, Hatakeyama S, Tanaka S, Abe T
  • Journal Title: RIKEN Accel Prog Rep Volume: 46
  • Peer Reviewed
• [Journal Article] A DFR-deficient mutant of Nicotiana tabacum induced by C-ion beam irradiation. (2013)
  • Author(s): Kazama Y, Fujiwara MT, Takehisa H, Ohbu S, Saito H, Ichida H, Hayashi Y, Abe T
  • Journal Title: RIKEN Accel Prog Rep. Volume: 46
  • Peer Reviewed
• [Journal Article] Characterization of a heavy-ion induced white flower mutant of allotetraploid Nicotiana tabacum. (2013)
  • Author(s): Kazama Y, Fujiwara MT, Takehisa H, Ohbu S, Saito H, Ichida H, Hayashi Y, Abe T.
  • Journal Title: Plant Cell Rep. Volume: 32 Pages: 11-19
  • DOI: DOI:10.1007/s00299-012-1336-7
  • Peer Reviewed
• [Journal Article] SlWUS1; An X-linked Gene Having No Homologous Y-Linked Copy in Silene latifolia. (2012)
  • Author(s): Kazama Y, Nishihara K, Bergero R, Fujiwara MT, Abe T, Charlesworth D, Kawano S
  • Journal Title: G3: Genes, Genomes, Genetics Volume: 2 Pages: 1269-1278
  • DOI: DOI:10.1534/g3.112.003749
  • Peer Reviewed
• [Journal Article] Rapid evaluation of effective linear energy transfer in heavy ion utagenesis of Arabidopsis thaliana. (2012)
  • Author(s): Kazama Y, Ma L, Hirano T, Ohbu S, Shirakawa Y, Hatakeyama S, Tanaka S, Abe T
  • Journal Title: Plant Biotechnology Volume: 29 Pages: 440-444
  • DOI: DOI:10.5511/plantbiotechnology.12.092 1a
  • Peer Reviewed
• [Journal Article] Narrowing down the mapping of plant sex-determination regions using new Y-chromosome-specific markers and heavy-ion beam irradiation-induced Y-deletion mutants in Silene latifolia. (2012)
  • Author(s): Fujita N, Torii C, Ishii K, Aonuma W, Shimizu Y, Kazama Y, Abe T, Kawano S.
  • Journal Title: G3: Genes, Genomes, Genetics Volume: 2 Pages: 271-278
  • DOI: DOI:10.1534/g3.111.001420/-/DC1
  • Peer Reviewed
• [Journal Article] A novel method of utilizing heavy-ion beams for efficient induction of large deletions in Arabidopsis thaliana. (2012)
  • Author(s): Hirano T, Kazama Y, Ohbu S, Shirakawa Y, Liu Y, Kambara T, Fukunishi N, Abe T
  • Journal Title: RIKEN Accel Prog Rep Volume: 45 Pages: 210
  • Peer Reviewed
• [Journal Article] Characterization of DNAs mutated by C-ion beam with highly efficient LET in mutagenesis of Arabidopsis thaliana. (2012)
  • Author(s): Kazama Y, Hirano T, Saito H, Liu Y, Ohbu S, Hayashi Y, Abe T
  • Journal Title: RIKEN Accel Prog Rep Volume: 45 Pages: 209
  • Peer Reviewed
• [Journal Article] Effect of linear energy transfer on deletion induction by irradiation with heavy-ion beams in the apomictic plant Panicum maximum. (2012)
  • Author(s): Takahara M, Ebina M, Morita R, Kazama Y, Abe T, Takamizo T, Nakagawa H
  • Journal Title: RIKEN Accel Prog Rep Volume: 45 Pages: 215
  • Peer Reviewed
• [Journal Article] Molecular nature of mutations induced by high-LET irradiation with argon and carbon ions in Arabidopsis thaliana (2012)
  • Author(s): Hirano T, Kazama Y, Ohbu S, Shirakawa Y, Liu Y, Kambara T, Fukunishi N, Abe T
  • Journal Title: Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis Volume: 735 Pages: 19-31
  • DOI: DOI:10.1016/j.mrfmmm.2012.04.010
  • Peer Reviewed
• [Journal Article] Visualization of plastid movement in the pollen tube of Arabidopsis thaliana (2012)
  • Author(s): Fujiwara M, Yoshioka Y, Hirano T, Kazama Y, Abe T, Hayashi K, Itoh R
  • Journal Title: Plant Signal Behav Volume: 7 Pages: 34-37
• [Journal Article] 重イオンビームによる品種改良技術の開発から遺伝子機能解明へ (2012)
  • Author(s): 阿部知子,平野智也,風間裕介
  • Journal Title: 日本物理学会誌 Volume: 67 Pages: 680-684
  • Peer Reviewed
• [Journal Article] Characterization of highly efficient heavy-ion mutagenesis in Arabidopsis thaliana. (2011)
  • Author(s): Kazama Y, Hirano T, Saito H, Liu Y, Ohbu S, Hayashi Y, Abe T
  • Journal Title: BMC Plant Biol Volume: 11 Pages: 161
  • DOI: DOI:10.1186/1471-2229-11-161
  • Peer Reviewed
• [Journal Article] Mutation frequency analysis of M_1 plants irradiated with heavy-ion beams. (2011)
  • Author(s): Kazama Y, Hirano T, Ohbu S, Shirakawa Y, Abe T
  • Journal Title: RIKEN Accel Prog Rep Volume: 44 Pages: 267
  • Peer Reviewed
• [Journal Article] Effect of LET on mutation induction by heavy-ion beam irradiation in imbibed seeds of Arabidopsis thaliana. (2011)
  • Author(s): Hirano T, Kazama Y, Ohbu S, Shirakawa Y, Abe T
  • Journal Title: RIKEN Accel Prog Rep Volume: 44 Pages: 268
  • Peer Reviewed
• [Journal Article] Production of bisexual flofwer-producing mutants of the dioecious plant, Silene latifolia, by using γ-ray and the heavy-ion. (2011)
  • Author(s): Aonuma W, Ishii K, Torii C, Fujita N, Shimizu Y, Nishihara K, Kazama Y, Abe T, Kawano S
  • Journal Title: RIKEN Prog Rep Volume: 44 Pages: 271
  • Peer Reviewed
• [Presentation] 重イオンビームを用いたシロイヌナズナタンデム遺伝子破壊ラインの構築 (2013)
  • Author(s): 風間裕介
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京
  • Year and Date: 2013-03-28
• [Presentation] 重イオンビームによるオンデマンド変異誘発法の開発とタンデム遺伝a子破壊ラインの構築 (2013)
  • Author(s): 風間裕介
  • Organizer: 日本植物生理学会第54回年会
  • Place of Presentation: 岡山
  • Year and Date: 2013-03-21
• [Presentation] Optimization of ion-beam irradiation for highly efficient mutagenesis using a rapid detection method of mutation (2012)
  • Author(s): Kazama Y
  • Organizer: EMBO Workshop: Genetic stability and change: Genome maintenance mechanisms in plants.
  • Place of Presentation: Roscoff, France
  • Year and Date: 20120503-04
• [Presentation] LET-dependent effects of heavy-ion irradiation on mutation induction in Arabidopsis thaliana (2012)
  • Author(s): Kazama Y
  • Organizer: 8th International Symposium on Swift Heavy Ions in Matter (SHIM 2012)
  • Place of Presentation: Kyoto
  • Year and Date: 2012-10-26
• [Presentation] ヒロハノマンテマ性染色体の組換え抑制によって生じたSlAP3X/Y 周辺の構造変化 (2012)
  • Author(s): 石井公太郎
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-15
• [Presentation] ヒロハノマンテマのSlAP3 欠損変異体を含むY 染色体部分欠損変異体の網羅的単離 (2012)
  • Author(s): 青沼航
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-15
• [Presentation] ヒロハノマンテマの2 つのホモログ遺伝子SlSUP とSlWUS の発現と性染色体座乗 (2012)
  • Author(s): 風間裕介
  • Organizer: 日本植物学会第76回大会
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-15
• [Presentation] 雌雄異株植物ヒロハノマンテマの効率的な染色体標本の作製 (2012)
  • Author(s): 石井公太郎
  • Organizer: 日本植物形態学会第24回総会・大会
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-14
• [Presentation] 重イオンビーム誘発性転換変異体を用いた植物Y 染色体性決定領域の解析 (2012)
  • Author(s): 風間裕介
  • Organizer: 新学術領域「ゲノム支援」2012 年度 拡大班会議
  • Place of Presentation: 御殿場
  • Year and Date: 2012-08-29
• [Presentation] 重イオンビーム照射によるアポミクシス遺伝子領域での欠失変異誘発と線量・LET の影響 (2012)
  • Author(s): 高原学
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮
  • Year and Date: 2012-03-30
• [Presentation] Ion-beam mutagenesis: New technology for the control of deletion-size by heavy-ion beam irradiation (2011)
  • Author(s): Kazama Y
  • Organizer: 18th International Botanical Congress (IBC2011)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20110723-30
• [Presentation] Ion beam mutagenesis: new innovative technology for mutation breeding (2011)
  • Author(s): Abe T
  • Organizer: 18th International Botanical Congress (IBC2011)
  • Place of Presentation: Melbourne, Australia
  • Year and Date: 20110723-30
• [Presentation] シロイヌナズナのM1 世代での迅速な突然変異検出系を用いたLET 効果の解析 (2011)
  • Author(s): 風間裕介
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井
  • Year and Date: 2011-09-24
• [Presentation] シロイヌナズナを用いたDNA 突然変異へのLET 効果の解析(2) (2011)
  • Author(s): 平野智也
  • Organizer: 日本育種学会第120回講演会
  • Place of Presentation: 福井
  • Year and Date: 2011-09-24
• [Presentation] ヒロハノマンテマX 染色体連鎖遺伝子SlWUS1 は性染色体進化の過程でY 染色体から消失した? (2011)
  • Author(s): 風間裕介
  • Organizer: 日本植物学会第75回大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-19
• [Presentation] アルビノ変異セクターを指標とした重イオンビーム変異迅速検出系の確立 (2011)
  • Author(s): 風間裕介
  • Organizer: 日本植物形態学会第23回総会・大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-16
• [Presentation] 重イオンビーム誘発突然変異に対するLET 効果の解析 (2011)
  • Author(s): 平野智也
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2011-09-07
• [Book] The role of repetitive sequences in the evolution of plant sex chromosomes, In : New Insights on Plant Sex Chromosomes (Rafael Navajas-Perez (ed)) (2012)
  • Author(s): Kazama Y, Matsunaga S
  • Total Pages: 21-34
  • Publisher: Nova Schience Publishers, Hauppauge, New York, U.S.A.
• [Remarks] (1)受賞風間裕介、平野智也、理研・研究奨励賞、2013年4月5日
• [Remarks] (2)アウトリーチ活動風間裕介、出前授業、「オスが子供を産む?重イオンビームで迫る植物Y染色体の不思議」、唐津東高校40名、2013年3月19日、唐津
• [Remarks] (3)アウトリーチ活動風間裕介、理研DAY:研究者と話そう、科学技術館、約40名、2013年3月17日、北の丸