Study on regulatory mechanisms of the expression and secretion of insulin-like peptides

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770071
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OKAMOTO Naoki 独立行政法人理化学研究所, 成長シグナル研究チーム, 基礎科学特別研究員 (10577969)
• Project Period (FY): 2011 – 2012
• Keywords: インスリン様ペプチド / インスリン産生細胞 / 成長 / ショウジョウバエ

Research Abstract

Regulatory mechanisms of the function of insulin-like peptides (ILPs) were analyzed by using fruit fly Drosophila melanogaster and mammalian pancreatic β cells-derived cell line. We found that the evolutionally conserved regulatory mechanism of the insulin expression by two transcriptional factors, Dachshund and Eyeless. We further characterized a novel ILPs-binding protein called secreted decoy of InR (SDR) that binds to ILPs in the circulating hemolymph and antagonizes insulin/IGF signaling during development.

Research Products

• [Journal Article] A secreted decoy of InR antagonizes insulin/IGF signaling to restrict body growth in Drosophila. (2013)
  • Author(s): Naoki Okamoto, Rinna Nakamori, Tomoka Murai, Yuki Yamauchi, Aya Masuda, Takashi Nishimura
  • Journal Title: Genes. Dev. Volume: 27 Pages: 87-97
  • Peer Reviewed
• [Journal Article] Conserved role for the Dachshund protein with Drosophila Pax6 homolog Eyeless in insulin expression. (2012)
  • Author(s): Naoki Okamoto, Yuka Nishimori, Takashi Nishimura
  • Journal Title: Proc. Natl. Acad. Sci. USA. Volume: 109 Pages: 2406-2411
  • Peer Reviewed
• [Presentation] Secreted decoy of InR antagonizes insulin signaling to restrict body growth in Drosophila (2013)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 2nd Psia-Pacific Drosophila Research Conference 2013
  • Place of Presentation: Seoul, South Korea
  • Year and Date: 2013-05-14
• [Presentation] ショウジョウバエ分泌型インスリン受容体は、体の成長を抑制する (2012)
  • Author(s): 岡本直樹,西村隆史
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 博多
  • Year and Date: 2012-12-12
• [Presentation] Secreted decoy of InR antagonizes insulin signaling to restrict body growth in Drosophila (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 日本ショウジョウバエ研究集会2012
  • Place of Presentation: 東京
  • Year and Date: 2012-10-15
• [Presentation] Regulatory mechanism of the Drosophila insulin-like peptide gene expression (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 日本ショウジョウバエ研究集会2012
  • Place of Presentation: 東京
  • Year and Date: 2012-10-14
• [Presentation] Regulatory mechanism of the Drosophila insulin-like peptides gene expression (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: New Frontiers of Metabolism Research in Biomedical Sciences
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2012-09-27
• [Presentation] Regulatory mechanism of the Drosophila insulin-like peptides gene expression (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 24th International Congress of Entomology 2012
  • Place of Presentation: Daegu, South Korea
  • Year and Date: 2012-08-24
• [Presentation] 昆虫インスリンの機能を調節するメカニズム (2012)
  • Author(s): 岡本直樹
  • Organizer: 2012 年度日本農芸化学会第1回例会シンポジウム「昆虫の行動を規定する化学
  • Place of Presentation: 東京
  • Year and Date: 2012-07-21
• [Presentation] Secreted decoy of InR antagonizes insulin signaling to restrict body growth in Drosophila (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 第46回 日本発生生物学会
  • Place of Presentation: 松江
  • Year and Date: 2012-05-31
• [Presentation] Regulation of the systemic growth by insulin-like peptides during Drosophila development (2012)
  • Author(s): Naoki Okamoto, Tomoka Murai, Takashi Nishimura
  • Organizer: 第45回 日本発生生物学会、第64回 日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-31
• [Presentation] Transcriptional regulation of Drosophila insulin-like peptides by nutrition (2012)
  • Author(s): Yuka Nishimori, Naoki Okamoto, Takashi Nishimura
  • Organizer: 第45回 日本発生生物学会、第64回 日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-31
• [Presentation] Conserved role for the Dachshund protein with Drosophila Pax6 homolog Eyeless in insulin expression (2012)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 第45回 日本発生生物学会、第64回 日本細胞生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2012-05-29
• [Presentation] 昆虫と哺乳類で保存されたインスリン発現制御機構 (2012)
  • Author(s): 岡本直樹
  • Organizer: 名古屋大学GCOE セミナー アドバンス生命理学持論
  • Place of Presentation: 名古屋
  • Year and Date: 2012-01-27
• [Presentation] An Evolutionarily Conserved Function of Dachshund/DACH to Regulate Insulin Expression Together with Eyeless/PAX6 (2011)
  • Author(s): Naoki Okamoto, Yuka Nishimori, Takashi Nishimura
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] 昆虫と哺乳類で保存されたDachshund/DACH によるインスリン様ペプチド発現制御機構 (2011)
  • Author(s): 岡本直樹,西村隆史
  • Organizer: 昆虫ワークショップ2011
  • Place of Presentation: 博多
  • Year and Date: 2011-10-13
• [Presentation] An Evolutionarily Conserved Function of Dachshund/DACH to Regulate Insulin Expression Together with Eyeless/PAX6, Exciting Biology Series - Cellular development (2011)
  • Author(s): Naoki Okamoto, Yuka Nishimori, Takashi Nishimura
  • Organizer: Biology at the interface
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2011-09-29
• [Presentation] A transgenic RNA interference screen for regulators of nutrient-dependent growth in the fruit fly Drosophila melanogaster (2011)
  • Author(s): Naoki Okamoto, Takashi Nishimura
  • Organizer: 第44回 日本発生生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2011-05-20
• [Book] むしむしコラム・おーどーこん -近くて不思議な虫の世界- : おとり受容体が体の成長を調節する (2013)
  • Author(s): 岡本直樹,西村隆史
  • Publisher: 日本応用動物昆虫学会電子広報委員会
• [Remarks]
  • URL: http://www.cdb.riken.jp/gcs/
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• [Remarks] ・読売新聞(夕刊)「成長制御するたんぱく質発見」(2013年1月15日)本業績に関する新聞記事