2012 Fiscal Year Final Research Report
Real-time imaging of excitation-secretioncoupling process from the single peptidergic neuron
| Project/Area Number |
23770072
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Animal physiology/Animal behavior
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| Research Institution | Nagoya University (2012)
The University of Tokyo (2011) |
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Principal Investigator |
ABE Hideki 名古屋大学, 大学院・生命農学研究科, 准教授 (90396804)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 神経生物 / ペプチド放出 / GnRH / 開口放出 / 興奮分泌連関 / 細胞培養 |
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| Research Abstract |
To understand fine tuning mechanisms of the central nervous system by peptidergic neurons, we investigated the excitation-secretion relationship of single peptidergic neuron using a primary culture system of isolated fish terminal nerve (TN)-gonadotropin releasing hormone (GnRH) neuron and gene transfer of exogenous genes by single cell electroporation. As a result, we enabled measurement of the movement of secretory vesicles and their exocytotic events from the subcellular region of single peptidergic neuron. We also analyzed the ionic mechanisms of the firing pattern control of TN-GnRH neurons that are the origin of exocytosis of GnRH peptide.
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Research Products
(29 results)
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[Journal Article] Time-of-Day-dependent changes in GnRH1 neuronal activities and gonadotropin mRNA expression in a daily spawning fish, medaka2012
Author(s)
Karigo, T., Kanda, S., Takahashi, A., Abe, H., Okubo, K., Oka, Y
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Journal Title
Endocrinology
Volume: 153
Pages: 3394-3404
Peer Reviewed
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[Presentation] eural mechanisms of female sexual preference in medaka2012
Author(s)
Okuyama, T., Yokoi, S., Abe, H., Taniguchi, Y., Okubo, K., Kamei, Y., Shimada, A., Naruse, K., Takeda, H., Oka, Y., Kubo, T., Takeuchi, H
Organizer
2nd Annual Meetings
Place of Presentation
Society for Neuroscience, New Orleans, USA
Year and Date
20121000
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[Presentation] Neural mechanisms of female sexual preference in medaka, Oryzias latipes (メダカにおける、メスの配偶者選択行動の神経機構)2012
Author(s)
奥山輝大, 横井佐織, 阿部秀樹, 末廣勇司, 今田はるか, 田中実, 川崎隆史, 谷口善仁, 亀井保博, 島田敦子, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
Organizer
第35回日本神経科学大会
Place of Presentation
名古屋
Year and Date
20120900
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[Presentation] 小型魚類を用いた視覚情報依存的な配偶者選択行動の分子・神経基盤解析2011
Author(s)
奥山輝大, 阿部秀樹, 末廣勇司, 今田はるか, 島田敦子, 川崎隆史, 弓場俊輔, 谷口善仁, 亀井保博, 田中実, 成瀬清, 武田洋幸, 岡良隆, 久保健雄, 竹内秀明
Organizer
第34回 日本分子生物学会年会
Place of Presentation
横浜
Year and Date
20111200
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[Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011
Author(s)
Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Abe, H., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
Organizer
1st medaka strategic meeting
Place of Presentation
Okazaki, JAPAN
Year and Date
20111100
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[Presentation] Analysis of regulatory mechanisms of gonadotropin secretion using transgenic medaka (Oryzias latipes)2011
Author(s)
Karigo, T., Aikawa, M., Kanda, S., Abe, H., Akazome,Y. Okubo, Y., and Oka, Y.
Organizer
41st Annual Meetings, Society for Neuroscience
Place of Presentation
Washington DC, USA
Year and Date
20111100
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[Presentation] Analysis of the molecular and neural mechanism of female mating acceptance depending on visual information in small fish medaka2011
Author(s)
Okuyama, T., Suehiro, Y., Imada, H., Shimada, A., Abe, H., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
Organizer
41st Annual Meetings, Society for Neuroscience
Place of Presentation
Washington DC, USA
Year and Date
20111100
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[Presentation] Analysis of the molecular and neural mechanism of female sexual preference that depends on visual information in small fish medaka2011
Author(s)
Okuyama, T., Abe, H., Suehiro, Y., Imada, H., Shimada, A., Kawasaki, T., Yuba, S., Taniguchi, Y., Kamei, Y., Tanaka, M., Naruse, K., Takeda, H., Oka, Y., Kubo, Y., and Takeuchi, H
Organizer
第17回小型魚類研究会
Place of Presentation
三島
Year and Date
20110900
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