Analysis of the diversity of fungal endophytes with metagenomics

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770083
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biodiversity/Systematics
• Research Institution: Kyoto University
• Principal Investigator: OSONO Takashi 京都大学, 生態学研究センター, 准教授 (90335307)
• Project Period (FY): 2011 – 2013
• Keywords: エンドファイト / メタゲノミクス / 多様性 / 林冠生物学 / 次世代シーケンサー / 熱帯林 / 菌類

Research Abstract

The alpha diversity and taxonomic composition of endophytic fungi associated with leaves of six tree species were examined in two lowland tropical forests and a subtropical forest in Asia and Australia, using ITS metabarcoding with Roche 454 pyrosequencing. A total of 95,278 fungal reads were clustered into 224 OTUs at 97% similarity threshold. Of the 178 OTUs identified to the phylum, 148 and 30 OTUs belonged to Ascomycota and Basidiomycota, respectively. Major OTUs were common foliar endophytes and pathogens. The low similarities of endophytic fungal assemblages between leaf samples of a single tree species suggested high beta diversity.

Research Products

• [Journal Article] Metagenomic approach yields insights into fungal diversity and functioning. In : Species Diversity and Community Structure : Novel Patterns and Processes in Plants, Insects, and Fungi. (2014)
  • Author(s): Osono T.
  • Journal Title: Springer Briefs in Biology (eds. By T. Sota et al.)( Springer, Berlin) Pages: 1-23
  • URL: http://link.springer.com/chapter/10.1007%2F978-4-431-54261-2_1
  • Peer Reviewed
• [Journal Article] Diversity and ecology of endophytic and epiphytic fungi of tree leaves in Japan : a review (2014)
  • Author(s): Osono T.
  • Journal Title: In : Advances in Endophytic Research (ed. by V.C. Verma and A.C. Gange)(Springer, Germany) Pages: 3-26
  • URL: http://link.springer.com/chapter/10.1007%2F978-81-322-1575-2_1
• [Journal Article] Comparison of the diversity, composition, and host recurrence of xylariaceous endophytes in subtropical, cool temperate, and subboreal regions in Japan (2014)
  • Author(s): Ikeda A., Matsuoka S., Masuya H., Mori A.S., Hirose D. & Osono T.
  • Journal Title: Population Ecology Volume: 56 Pages: 289-300
  • URL: http://link.springer.com/article/10.1007/s10144-013-0412-3
  • Peer Reviewed
• [Journal Article] Assessment of the fungal diversity and succession of ligninolytic endophytes in Camellia japonica leaves using clone library analysis (2013)
  • Author(s): Hirose D., Matsuoka S. & Osono T.
  • Journal Title: Mycologia Volume: 105 Pages: 837-843
  • Peer Reviewed
• [Journal Article] Microfungi associated with withering willow wood in ground contact near Syowa Station, East Antarctica for 40 years (2013)
  • Author(s): Hirose D., Tanabe Y., Uchida M., Kudoh & Osono T.
  • Journal Title: Polar Biology Volume: 36 Pages: 919-924
  • URL: http://www.mycologia.org/content/105/4/837.abstract
  • Peer Reviewed
• [Journal Article] Microfungi associated with a myrmecophyte Macaranga bancana (2013)
  • Author(s): Hirose D., Sakai S., Itioka T. & Osono
  • Journal Title: Tropics Volume: 22 Pages: 19-25
  • URL: https://www.jstage.jst.go.jp/article/tropics/22/1/22_19/_article/-char/ja/
  • Peer Reviewed
• [Journal Article] Diversity and ubiquity of xylariaceous endophytes in live and dead leaves of temperate forest trees (2013)
  • Author(s): Osono T., Tateno O. & Masuya H.
  • Journal Title: Mycoscience Volume: 54 Pages: 54-61
  • URL: http://www.sciencedirect.com/science/article/pii/S1340354012000125
  • Peer Reviewed
• [Journal Article] Biodiversity of fungi in a tropical rain forest. Eds by Sakai S., Ishii R. & Yamamura N. Collapse and Restoration of Ecosystem Networks with Human Activity (2013)
  • Author(s): Osono T., Hirose D., Nagamasu H. & Itioka T.
  • Journal Title: RIHN Project Report Volume: (Published by Research Institute for Humanity and Nature) Pages: 137-140
• [Journal Article] Endophytic fungi associated with leaves of Betulaceae in Japan (2012)
  • Author(s): Osono T. & Masuya H.
  • Journal Title: Canadian Journal of Microbiology Volume: 58 Pages: 507-515
  • Peer Reviewed
• [Journal Article] Endophytic fungal assemblages on leaves of 73 deciduous tree species in a cool temperate forest (2012)
  • Author(s): Osono T.
  • Journal Title: Applied Forest Science Volume: 21 Pages: 13-20
  • URL: http://www.nrcresearchpress.com/doi/abs/10.1139/w2012-018#.U2CA2HMspaI
  • Peer Reviewed
• [Journal Article] Colonization and lignin decomposition of pine needle litter by Lophodermium pinastri (2011)
  • Author(s): Osono T. & Hirose D.
  • Journal Title: Forest Pathology Volume: 41 Pages: 156-162
  • DOI: 10.1111/j.1439-0329.2010.00648.x/abstract
  • Peer Reviewed
• [Presentation] 知床羅臼岳の針広混交林における樹木葉内生菌の標高分布とその制限要因 (2013)
  • Author(s): 阪口瀬理奈・松岡俊将・川口恵里・森章・広瀬大・大園享司
  • Organizer: 日本菌学会第57回大会
  • Place of Presentation: 東京農業大学, 東京
  • Year and Date: 20130600
• [Presentation] 知床羅臼岳の標高傾度に沿った内生菌類群集の変化 (2013)
  • Author(s): 阪口瀬理奈・松岡俊将・川口恵里・森章・大園享司
  • Organizer: 日本生態学会第60回大会
  • Place of Presentation: グランシップ静岡(ポスター講演)
  • Year and Date: 20130300
• [Presentation] 次世代シーケンサーを用いたアジア熱帯林における内生菌類の群集構造の比較 (2012)
  • Author(s): 阪口瀬理奈・松岡俊将・伊藤公一・広瀬大・矢澤重信・西村理・田邊晶文・東樹宏和・大園享司
  • Organizer: 日本菌学会第56回大会
  • Place of Presentation: 岐阜大学, 岐阜
  • Year and Date: 20120500
• [Presentation] Next-generation sequencing reveals the hyper-diversity of endophytic fungi associated with tree leaves in tropical forests (2012)
  • Author(s): Sakaguchi C, Matsuoka S, Ito K, Hirose D, Yazawa S, Nishimura O, Tanabe A, Toju H, Osono T.
  • Organizer: The 5th East Asian Federation of Ecological Societies (EAFES) International Congress
  • Place of Presentation: Ryukoku University, Shiga, Japan (poster session)
  • Year and Date: 20120300
• [Presentation] Diversity of Xylariaceous fungi on living and dead leaves : endophytes become saprophytes? (2012)
  • Author(s): Ikeda A, Hirose D, Matsuoka S, Osono T.
  • Organizer: The 5th East Asian Federation of Ecological Societies (EAFES) International Congress
  • Place of Presentation: Ryukoku University, Shiga, Japan (poster session)
  • Year and Date: 20120300
• [Presentation] 次世代シーケンサーで明らかにする亜熱帯林内生菌の超多様性 (2011)
  • Author(s): 阪口瀬理奈・松岡俊将・伊藤公一・広瀬大・矢澤重信・西村理・大園享司
  • Organizer: 日本菌学会第55回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 20110900
• [Presentation] カバノキ科樹木の葉に見いだされる菌類エンドファイトの多様性 (2011)
  • Author(s): 大園享司・升屋勇人
  • Organizer: 日本菌学会第55回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 20110900
• [Presentation] 国内亜熱帯林におけるクロサイワイタケ科内生菌の多様性―気候帯間比較による評価 (2011)
  • Author(s): 池田あんず・広瀬大・松岡俊将・大園享司
  • Organizer: 日本菌学会第55回大会
  • Place of Presentation: 北海道大学, 札幌
  • Year and Date: 20110900
• [Presentation] Diversity and host-specificity of endophytic Xylariaceae in a subtropical forest in Japan inferred from rDNA sequence analysis (2011)
  • Author(s): Ikeda A, Hirose D, Matsuoka S, Osono T.
  • Organizer: XIII International Congress of Mycology (IUMS2011 Sapporo)
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan. (poster session)
  • Year and Date: 20110900
• [Presentation] 454 sequencing reveals the hyper-diversity of endophytic fungi associated with tree leaves in a subtropical forest in southern Japan (2011)
  • Author(s): Sakaguchi C, Matsuoka S, Ito K, Hirose D, Yazawa S, Nishimura O, Osono T.
  • Organizer: XIII International Congress of Mycology (IUMS2011 Sapporo)
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan. (poster session)
  • Year and Date: 20110900
• [Presentation] Environmental DNA analysis reveals the fungal succession on Camellia japonica leaves and the functioning of ligninolytic endophytes (2011)
  • Author(s): Osono T, Hirose D.
  • Organizer: XIII International Congress of Mycology (IUMS2011 Sapporo)
  • Place of Presentation: Sapporo Convention Center, Sapporo, Japan. (poster session)
  • Year and Date: 20110900
• [Presentation] Metagenomics of hyper-diversity of tropical fungi (2011)
  • Author(s): Osono T.
  • Organizer: 日本進化学会2011京都シンポジウム「未知の多様性探索とゲノム情報」
  • Place of Presentation: 京都大学, 京都
  • Year and Date: 20110700
• [Presentation] Next-generation sequencing reveals the hyper-diversity of endophytic fungi associated with tree leaves in a subtropical forest in southern Japan (2011)
  • Author(s): Sakaguchi C, Matsuoka S, Ito K, Hirose D, Yazawa S, Nishimura O, Osono T.
  • Organizer: The 5th International Symposium of the Biodiversity & Evolution gCOE
  • Place of Presentation: Kyoto University, Kyoto, Japan. (poster session)
  • Year and Date: 20110700
• [Presentation] The diversity of fungi in ant-plants and a three-way symbiosis (2011)
  • Author(s): Nagao Y, Hirose D, Osono T.
  • Organizer: The 5th International Symposium of the Biodiversity & Evolution gCOE
  • Place of Presentation: Kyoto University, Kyoto, Japan. (poster session)
  • Year and Date: 20110700
• [Presentation] Phylogenetic diversity and host specificity of Coccomyces in Asian forests (2011)
  • Author(s): Osono T, Hirose D.
  • Organizer: The 5th International Symposium of the Biodiversity & Evolution gCOE
  • Place of Presentation: Kyoto University, Kyoto, Japan. (poster session)
  • Year and Date: 20110700
• [Presentation] The diversity of Xylariaceous endophytes in a subtropical forest in Japan : comparison with different climate zones (2011)
  • Author(s): Ikeda A, Hirose D, Matsuoka S, Osono T.
  • Organizer: The 5th International Symposium of the Biodiversity & Evolution gCOE
  • Place of Presentation: Kyoto University, Kyoto, Japan. (poster session)
  • Year and Date: 20110700
• [Presentation] 亜熱帯林における内生菌の種多様性と宿主特異性 (2011)
  • Author(s): 池田あんず・広瀬大・松岡俊将・大園享司
  • Organizer: 京都大学微生物科学寄付研究部門主催シンポジウム「微生物科学研究の現状と展望」(ポスター講演)
  • Place of Presentation: 京都大学, 京都
  • Year and Date: 20110600
• [Presentation] 次世代シケンサーで解析する亜熱帯林内生菌の超多様性 (2011)
  • Author(s): 阪口瀬理奈・松岡俊将・伊藤公一・広瀬大・矢澤重信・西村理・大園享司
  • Organizer: 京都大学微生物科学寄付研究部門主催シンポジウム「微生物科学研究の現状と展望」(ポスター講演)
  • Place of Presentation: 京都大学, 京都
  • Year and Date: 20110600
• [Presentation] 漂白化した落葉上に子実体形成するCoccomyces属菌の系統的多様性と宿主特異性 (2011)
  • Author(s): 広瀬大・大園享司
  • Organizer: 平成23年度日本菌学会関東支部会年次大会
  • Place of Presentation: 東京農業大学, 東京
  • Year and Date: 20110400
• [Book] 熱帯林・亜熱帯林の落ち葉は白く腐る.生き物たちのつづれ織り(下)(阿形清和・森哲監修、高林純示ほか編) (2012)
  • Author(s): 大園享司
  • Total Pages: 139-149
  • Publisher: 京都大学学術出版会
• [Book] 分解.森のバランス―植物と土壌の相互作用(森林立地学会編) (2012)
  • Author(s): 大園享司
  • Total Pages: 187-196
  • Publisher: 東海大学出版会
• [Book] グラスエンドファイト―その生態と進化(原題:Ecology and Evolution of the Grass-Endophyte Symbiosis, Cheplick G.P. & Faeth S.H.) (2012)
  • Author(s): 大園享司(訳)
  • Publisher: 東海大学出版会
• [Book] 菌類の生物学(原題:Fungal Biology, Understanding the fungal lifestyle, second edition, Lysek G. & Jennings D.H.) (2011)
  • Author(s): 広瀬大・大園享司(訳)
  • Publisher: 京都大学学術出版会
• [Book] 鏡味麻衣子生態学からみた微生物の世界.微生物の生態学(大園享司・鏡味麻衣子編) (2011)
  • Author(s): 大園享司
  • Total Pages: 3-15
  • Publisher: 共立出版
• [Book] 鏡味麻衣子微生物の生態学 (2011)
  • Author(s): 大園享司
  • Publisher: 共立出版
• [Remarks] 2011年10月2日 ひらめき☆ときめきサイエンス～ようこそ大学の研究室へ～KAKENHI「みんなちがうのにいっしょにくらしている生態系の不思議:生物の多様性って何だろう?」実施分担者, アウトリーチ活動
• [Remarks] 2012年11月18日 サイエンスカフェ高槻「みんなの知らない南極のカビ・きのこの話」講師, アウトリーチ活動
• [Remarks]
  • URL: http://www.ecology.kyoto-u.ac.jp/~tosono/