Molecular recognition of HLA-G6 isoform with LILR family

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770102
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Hokkaido University
• Principal Investigator: KUROKI Kimiko 北海道大学, 大学院・薬学研究院, 助教 (90553313)
• Project Period (FY): 2011 – 2012
• Keywords: HLA-G6 / 構造解析 / 相互作用解析

Research Abstract

I performed the analysis at the protein level about the interaction with HLA-G6 isoform and the LILR receptor. At first I established the expression system of stable HLA-G6 protein by E.coli. In addition, I performed structural analyses of HLA-G6 and the complex with its receptor, LILRB2, by several techniques (X-ray crystallography, cryo-EM, NMR).

Research Products

• [Journal Article] Structural and functional mosaic nature of MHC class I molecules in their peptide-free form. (2013)
  • Author(s): Kurimoto E, Kuroki K, Yamaguchi Y,Yagi-Utsumi M, Igaki T, Iguchi T, MaenakaK, Kato K.
  • Journal Title: Mol Immunol. Volume: 55(3-4) Pages: 393-9
  • DOI: doi:10.1016/j.molimm.2013.03.014.
  • Peer Reviewed
• [Journal Article] The long-term immunosuppressive effects of disulfide-linked HLA-G dimer in mice with collagen-induced arthritis. (2013)
  • Author(s): Kuroki K, Hirose K, Okabe Y, Fukunaga Y, Takahashi A, Shiroishi M, Kajikawa M, Tabata S, Nakamura S, Takai T, Koyanagi S, Ohdo S, Maenaka K.
  • Journal Title: Hum Immunol. Volume: 74(4) Pages: 433-8
  • DOI: doi:10.1016/j.humimm.2012.11.060.
  • Peer Reviewed
• [Presentation] ヒト免疫制御受容体LILRB1とリガンドHLA-Gの複合体の結晶構造解析 (2012)
  • Author(s): 黒木喜美子
  • Organizer: 分子生物学会
  • Place of Presentation: 福岡・福岡国際会議場・マリンメッセ福岡
  • Year and Date: 20121211-14
• [Presentation] Structural basis for recognition of nonclassical MHC molecule HLA-G by the LILRB1 (2012)
  • Author(s): Kimiko Kuroki
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: The Alumni Hall "Frate", Graduate School of Medicine, Hokkaido University
  • Year and Date: 20121002-03
• [Presentation] Structural basis for recognition of nonclassical MHC molecule HLA-G by the LILRB1 (2012)
  • Author(s): Haruki Matsubara
  • Organizer: 6th International conference on HLA-G
  • Place of Presentation: Paris, Fondation Del Duca
  • Year and Date: 20120907-11
• [Presentation] Immunosuppressive effects of treatment with HLA-G dimer in collagen-induced mouse arthritis (2012)
  • Author(s): Kimiko Kuroki
  • Organizer: 6th International conference on HLA-G
  • Place of Presentation: Paris, Fondation Del Duca
  • Year and Date: 20120709-11
• [Presentation] 免疫抑制分子HLA-Gダイマーの局所投与による関節炎抑制効果の検討 (2011)
  • Author(s): 黒木喜美子
  • Organizer: 分子生物学会
  • Place of Presentation: 横浜・パシフィコ横浜
  • Year and Date: 20111213-16