2012 Fiscal Year Final Research Report
Structural analysis of the Rab and SNARE localization
| Project/Area Number |
23770107
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | The University of Tokyo |
|---|
Principal Investigator |
ITO Sakurako 東京大学, 放射光連携研究機構, 特任助教 (60597152)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | X線結晶構造解析 |
|---|
| Research Abstract |
In eukaryotic cells, Rab and SNARE regulate the fusion of membrane compartments. Rabs are inseted into membranes via the prenylated moieties which are post-translationally attached. A membrane protein called GDF is proposed to catalyze the membrane insertion of Rab. As GDF and VAMP2, one of the SNARE protein, is shown to interact with each other, we are trying to reveal the structural basis of GDF-SNARE cooperation. So far, we have succeeded in the crystallization of GDF, and now trying to improve thediffraction quality of crystals. Also, we are trying to prepare GDF-VAMP2 complex.
|
