2012 Fiscal Year Final Research Report
The development of a method for studying aromatic ring flipping motion in the interior of proteins
| Project/Area Number |
23770109
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | NMR / SAIL |
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| Research Abstract |
Aromatic rings of phenylalanine and tyrosine residues in the interior of proteins infrequently rotates about their Cb-Cg axis in concert with the large amplitude slow breathing motion of protein molecules. An NMR method for evaluating ring-flipping rate based on a Carr-Purcell Meiboom Gill relaxation experiments and SAIL-NMR method is developed. This method enables the quantitative evaluation of the ring-flipping phenomenon on the micro-second time scales.
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