2013 Fiscal Year Final Research Report

Biological Solid-State NMR for Challenging Samples - Sensitivity Enhancement using Visible Light

Research Project

Project/Area Number	23770114
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Structural biochemistry
Research Institution	Osaka University
Principal Investigator	MATSUKI Yoh 大阪大学, たんぱく質研究所, 助教 (70551498)
Project Period (FY)	2011 – 2013
Keywords	固体NMR / 感度向上 / 光化学誘起動的核分極
Research Abstract	Instruments and fundamental techniques have been developed toward establishing a general way for enhancing sensitivity of solid-state NMR via nuclear hyperpolarization based on photo excited dye molecules: photo-chemically induced dynamic nuclear polarization (CIDNP). Using newly developed photo-sensitizer system using flavin derivatives and imidazole embedded in a micelle, enhancement of net nuclear polarization via photo-CIDNP has been observed. It was also found that paramagnetic relaxation enhancement (PRE) effect of the photo-excited dye molecules significantly accelerates the longitudinal relaxation rate of bulk nuclear polarization, enabling rapid signal accumulation or unit-time sensitivity enhancement. The above methods based on light-excited dye overcome a major drawbacks of conventional DNP and PRE techniques: paramagnetic signal broadening, because the paramagnetic species can be quenched by turning the light illumination off during signal acquisition.

(2 results)