Structural study of telomere maintenance mechanism of RecQ helicases

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KITANO Ken 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (40346309)
• Project Period (FY): 2011 – 2012
• Keywords: タンパク質 / DNA ヘリカーゼ / RecQ ファミリー / Ｘ線結晶解析 / 構造生物学 / 生物物理学 / 三量体Ｇタンパク質 / 阻害剤

Research Abstract

RecQ family of DNA helicases WRN (Werner syndrome protein) and BLM (Bloom syndrome protein) play a key role in protecting the genome against deleterious changes. In this study, we determined the first three-dimensional structure of a RecQ C-terminal (RQC) domain from human BLM bound to a phosphate ion. Our result gave insights into the unique DNA-unwinding activity of RecQ helicases toward recombination and repair intermediates (Kim, SY., Hakoshima, T., and Kitano, K., 2013, Sci. Rep., 3, 3294.).

Research Products

• [Journal Article] ヒトRecQヘリカーゼWRNとBLMの結晶構造解析 (2014)
  • Author(s): 北野健
  • Journal Title: 日本結晶学会誌 Volume: 56(2) Pages: 133-138
  • DOI: 10.5940/jcrsj.56.133
  • Peer Reviewed
• [Journal Article] Structure of the RecQ C- terminal domain of human Bloom syndrome protein (2013)
  • Author(s): Kim, SY., Hakoshima, T., Kitano, K.
  • Journal Title: Scientific Reports Volume: 3 Pages: 3294
  • DOI: 10.1038/srep03294
  • Peer Reviewed
• [Presentation] Structural study of Bloom syndrome protein (2014)
  • Author(s): Kim, SY., Hakoshima, T., Kitano, K.
  • Organizer: Biophysical Society 58th Annual Meeting
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2014-02-18
• [Presentation] RadixinとMT1-MMPとの複合体のX線結晶構造解析 (2013)
  • Author(s): 寺脇慎一,北野健,森智行,青山美樹,箱嶋敏雄
  • Organizer: 平成25年度日本結晶学会年会
  • Place of Presentation: 熊本大学,熊本市
  • Year and Date: 2013-10-12
• [Presentation] Protein interaction between NADH oxidase and AhpC (Prx) from Amphibacillus xylanus (2013)
  • Author(s): Mochizuki, D., Arai, T., Hara, K., Matsumoto, T., Kitano, K., Zako, T., Okada, M., Yohda, M., Sato, J., Kawasaki, S., Kanamaru, S., Arisaka, F., Niimura, Y.
  • Organizer: 21st International Conference on Analytical Ultracentrifugation, Hydrodynamics, Thermodynamics and Complementary Methods (AUC2013)
  • Place of Presentation: 静岡県熱海市,ホテルニューアカオ
  • Year and Date: 2013-09-26
• [Presentation] 膜貫通型プロテアーゼMT1-MMPのERM蛋白質による細胞骨格連結の構造的基盤 (2013)
  • Author(s): 寺脇慎一,北野健,青山美樹,箱嶋敏雄
  • Organizer: 第13回日本蛋白質科学会年会
  • Place of Presentation: とりぎん文化会館,鳥取市
  • Year and Date: 2013-06-13
• [Presentation] Allosteric inhibition of heterotrimeric Gq protein by a small molecule (2013)
  • Author(s): Kitano, K., Nishimura, A., Hakoshima, T., Itoh, H.
  • Organizer: EMBO Conference on Allosteric Interactions in Cell Signaling and Regulation
  • Place of Presentation: Paris, France
  • Year and Date: 2013-05-15
• [Presentation] Crystallographic Studies of Werner Syndrome DNA Helicase (2012)
  • Author(s): Kitano, K., Kim, S.Y., Hakoshima, T.
  • Organizer: Meeting of the American Crystallographic Association (ACA2012)
  • Place of Presentation: Boston, USA
  • Year and Date: 2012-07-29
• [Presentation] ERM蛋白質による膜貫通型プロテアーゼMT1-MMP認識の構造的基盤 (2012)
  • Author(s): 寺脇慎一,北野健,青山美樹,箱嶋敏雄
  • Organizer: 日本生化学会関東支部例会
  • Place of Presentation: 群馬大学,前橋市
  • Year and Date: 2012-06-23
• [Presentation] 環状ペプチドによる三量体Gタンパク質の阻害機構 (2011)
  • Author(s): 北野健
  • Organizer: 日本学術振興会169委員会第37回研究会
  • Place of Presentation: ゆうぽうと,東京
  • Year and Date: 2011-12-13
• [Presentation] 三量体Gタンパク質の構造生物学と環状ペプチドのドラッグデザイン (2011)
  • Author(s): 北野健
  • Organizer: 包括脳ネットワーク研究会・蛋白研セミナー共催セミナー
  • Place of Presentation: 岡崎コンファレンスセンター
  • Year and Date: 2011-11-22
• [Presentation] Structural study of Werner syndrome DNA helicase (2011)
  • Author(s): Kitano, K., Kim, S.Y., Hakoshima, T.
  • Organizer: The EMBO Meeting
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2011-09-12
• [Presentation] Structural biology of human RecQ DNA helicases (2011)
  • Author(s): Kitano, K.
  • Organizer: The 2nd International Conference on Pharmacy and Advanced Pharmaceutical Sciences
  • Place of Presentation: Yogyakarta, Indonesia
  • Year and Date: 2011-07-19
• [Presentation] 微小管+端集積因子,EB1-CLASPの複合体結晶構造解析 (2011)
  • Author(s): 森智行,北野健,箱嶋敏雄
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: ホテル阪急エキスポパーク,大阪
  • Year and Date: 2011-06-07
• [Remarks] Protein Data Bank (PDB)への登録 ①Kim, SY., Hakoshima, T., Kitano, K. (2013), "Structure of BLM RQC domain bound to a phosphate ion." 3WE2.