Structural study of slective cargo transport by unconventional myosins

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770119
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HIRANO Yoshinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529)
• Project Period (FY): 2011 – 2012
• Keywords: X線結晶構造解析 / 分子認識 / 積荷輸送 / ミオシン / 微小管

Research Abstract

The Cargo transportation by myosins is essential for proper cell phenomena. We peformed a series of structural and biochemicalstudies of the myosin-X tail domain , consisting of MyTH4 and FERM domains in complex with its specific cargo, a netrin receptor DCC (deleted in colorectal cancer). As the result, we found an unexpected binding mode of the DCC peptide, which is distinct from previously reported associations found in radixin/adhesion molecule complexes.
Moreover, we revealed direct interactions between the MyTH4-FERM domain and tubulin C-terminal acidic tails, and identified a positively charged patch of the MyTH4 domain as a tubulin binding region. We also demonstrated that both DCC and integrin bindings interfere with microtubule binding and that DCC binding interferes with integrin binding. Our results provide the molecular basis by which myosin-X facilitates alternative dual binding to cargos and microtubules.

Research Products

• [Journal Article] Mechanism of high-affinity abscisic acid binding to PYL9/RCAR1 (2014)
  • Author(s): Nakagawa M, Kagiyama M, Shibata N, Hirano Y, Hakoshima T
  • Journal Title: Genes to Cells Volume: 19 Pages: 386-404
  • Peer Reviewed
• [Journal Article] Structural and functional analysis of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance via proline metabolism (2013)
  • Author(s): Nasuno, R., Hirano, Y., Itoh, T., Hakoshima, T., Hibi, T., Takagi, H
  • Journal Title: Proceedings of the National Academy of Sciences of the U.S.A. Volume: 110 Pages: 11821-11826
  • Peer Reviewed
• [Journal Article] (2013)
  • Author(s): Kagiyama, M., Hirano, Y., Mori, T., Kim, S.Y., Kyozuka, J., Seto, Y., Yamaguchi, S., Hakoshima, T
  • Journal Title: Genes to Cells Volume: 18 Pages: 147-160
  • Peer Reviewed
• [Journal Article] 植物ホルモンによって誘導されるタンパク質分解のメカニズム (2011)
  • Author(s): 村瀬浩司,平野良憲,箱嶋敏雄
  • Journal Title: 実験医学 Volume: 29 Pages: 1987-1994
• [Journal Article] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain (2011)
  • Author(s): Hirano,Y., Hatano,T., Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T
  • Journal Title: EMBO J Volume: 30 Pages: 2734-2747
  • Peer Reviewed
• [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析 (2013)
  • Author(s): 那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
  • Organizer: 酵母遺伝学フォーラム第46回研究報告会
  • Place of Presentation: 宮城県仙台市東北学院大学土樋キャンパス8号館
  • Year and Date: 2013-09-08
• [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析 (2013)
  • Author(s): 那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
  • Organizer: 第13回食品酵素科学研究会
  • Place of Presentation: 大阪府大阪市大阪府立大学I-site難波
  • Year and Date: 2013-09-02
• [Presentation] Structural and functional analyses of the yeast N-acetyltransferase Mpr1 involved in oxidative stress tolerance (2013)
  • Author(s): 那須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 宮城県仙台市
  • Year and Date: 2013-03-25
• [Presentation] 酵母の酸化ストレス耐性に関与するN-アセチルトランスフェラーゼMpr1の構造機能解析 (2012)
  • Author(s): 須野亮,平野良憲,伊藤貴文,箱嶋敏雄,日び隆雄,高木博史
  • Organizer: 第85会日本生化学会大会
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2012-12-15
• [Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain (2011)
  • Author(s): Hirano,Y., Hatano,T, Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T.
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: マドリッド(スペイン)
  • Year and Date: 2011-08-24
• [Presentation] Myosin-XのMyTH4-FERMドメインによる積み荷認識機構の構造的基盤 (2011)
  • Author(s): 平野良憲,畑野大樹,高橋彩,箱嶋敏雄
  • Organizer: 第11回日本蛋白質科学会年会
  • Place of Presentation: 大阪府吹田市ホテル阪急エキスポパーク
  • Year and Date: 2011-06-07