2012 Fiscal Year Final Research Report
Reverse Glycomic Approach for Development of Simple Diagnostic Method of Antitumor-Drug Resistance
| Project/Area Number |
23770120
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Structural biochemistry
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
NAKANO Miyako 広島大学, 先端物質科学研究科, 准教授 (40397724)
|
|---|
| Project Period (FY) |
2011 – 2012
|
| Keywords | 糖質 |
|---|
| Research Abstract |
To develop simple diagnostic method of antitumor-drug resistance, we determined the changes in glycosylation that occur on membrane proteins of drug-resistant T-cell acute lymphoblastic leukemia cell line. Drug-resistant cells showed a significant decrease in sialylated glycans. To ascertain if identical alterations occurred on leukemia cell in the blood of leukemia patients, the analytical conditions for LC-ESI MS of glycan structures were investigated. We established the conditions that could analyze their glycan structures using 3 mL of blood. Moreover, we showed that the lectin recognized sialic acids was useful for investigating a simple diagnostic method of drug-resistance acquisition.
|
