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2012 Fiscal Year Final Research Report

Structural study on the generation mechanism of nitrous oxide by integral membrane protein nitric oxide reductase

Research Project

  • PDF
Project/Area Number 23770144
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionTottori University

Principal Investigator

HINO Tomoya  鳥取大学, 大学院・工学研究科, 講師 (40373360)

Project Period (FY) 2011 – 2012
Keywords金属タンパク質 / 膜蛋白質
Research Abstract

Bacterial nitric oxide reductase is a membrane integral enzyme that catalyzes the reduction of nitric oxide to nitrous oxide during the nitrate respiration, an anaerobic respiration of facultative anaerobic organisms. In the present study, I determined the crystal structure of nitric oxide reductase from Pseudomonas aeruginosa in the fully reduced state and revealed that no obvious structural changes were induced including active site upon reduction of all metal sites

  • Research Products

    (16 results)

All 2013 2012 2011 Other

All Journal Article (9 results) (of which Peer Reviewed: 6 results) Presentation (4 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Molecular dynamics simulations reveal proton transfer pathways in cytochrome c-dependent nitric oxide reductase2012

    • Author(s)
      Pisliakov A. V., Hino T., Shiro Y., Sugita Y.
    • Journal Title

      PLoS Comput.

      Volume: 8(8) Pages: e1002674

    • Peer Reviewed
  • [Journal Article] G-protein coupled receptor inactivation by an allosteric inverse-agonist antibody2012

    • Author(s)
      Hino T., Arakawa H., Iwanari H., Yurugi-Kobayashi T., Ikeda-Suno C., Nakada-Nakura Y., Kusano-Arai O., Weyand S., Shimamura T., Nomura N., Cameron A. D., Kobayashi T., Hamakubo T., Iwata S., Murata T.
    • Journal Title

      Nature

      Volume: 482 Pages: 237-240

    • Peer Reviewed
  • [Journal Article] Crystal Structure of Quinol-dependent Nitric Oxide Reductase from Geobacillus Stearothermophilus2012

    • Author(s)
      Matsumoto Y., Tosha T., Pisliakov A. V., Hino T., Sugimoto H., Nagano S., Sugita Y., Shiro Y.
    • Journal Title

      Nature Struct. Mol. Biol.

      Volume: 19 Pages: 238-245

    • Peer Reviewed
  • [Journal Article] Molecular Structure and Function of Bacterial Nitric Oxide Reductase2012

    • Author(s)
      Hino T., Nagano S., Sugimoto H., Tosha T., Shiro Y.
    • Journal Title

      Biochem. Biophys. Acta Bioenergetics

      Volume: 1817 Pages: 680-687

    • Peer Reviewed
  • [Journal Article]2012

    • Author(s)
      Shiro Y., Sugimoto H., Tosha T., Nagano S., Hino T.
    • Journal Title

      Philos. Trans. Soc. Lond. B Biol. Sci.

      Volume: 367 Pages: 1195-1203

    • Peer Reviewed
  • [Journal Article] アロステリックな逆作動薬としての活性をもつ機能性抗体によるGタンパク質共役型受容体の不活性化の分子機構2012

    • Author(s)
      日野智也、岩田想、村田武士
    • Journal Title

      新着論文レビュー

      Volume: 4560

  • [Journal Article] 抗体と膜タンパク質の共結晶構造2012

    • Author(s)
      日野智也、岩田想、村田武士
    • Journal Title

      新機能抗体開発ハンドブック

      Volume: 第5章-3

  • [Journal Article] 一酸化窒素還元酵素の結晶構造から見えてきた呼吸酵素の機能変換2012

    • Author(s)
      日野智也、當舎武彦、城宜嗣
    • Journal Title

      生物物理

      Volume: 52 Pages: 186-189

    • Peer Reviewed
  • [Journal Article] 機能性抗体による創薬標的Gタンパク質共役型受容体の不活性化の分子機構2012

    • Author(s)
      日野智也、岩田想、村田武士
    • Journal Title

      実験医学

      Volume: 30 Pages: 186-189

  • [Presentation] 膜タンパク質結晶化促進ツールとしての抗体利用2012

    • Author(s)
      日野智也
    • Organizer
      日本生物工学会西日本支部シンポジウム
    • Place of Presentation
      鳥取
    • Year and Date
      2012-01-21
  • [Presentation] 緑膿菌由来膜タンパク質一酸化窒素還元酵素の抗体を用いた結晶化と構造解析2011

    • Author(s)
      日野智也
    • Organizer
      日本生化学会 第84回年会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
  • [Presentation] 一酸化窒素還元酵素の立体構造から探る呼吸酵素の分子進化2011

    • Author(s)
      日野智也
    • Organizer
      日本生物物理学会 第49回年会
    • Place of Presentation
      姫路
    • Year and Date
      2011-09-16
  • [Presentation] 緑膿菌由来一酸化窒素還元酵素の結晶構造解析2011

    • Author(s)
      日野智也
    • Organizer
      日本蛋白質科学会 第11回年会
    • Place of Presentation
      大阪
    • Year and Date
      2011-06-08
  • [Remarks]

    • URL

      http://www.bio.tottori-u.ac.jp/~bioeng/

  • [Patent(Industrial Property Rights)] 芳香族スルホン酸化合物を用いた新規Clear Native電気泳動法2013

    • Inventor(s)
      日野智也
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構、村田武士
    • Industrial Property Number
      特許第5213967号
    • Acquisition Date
      2013-03-08
  • [Patent(Industrial Property Rights)] アゴニストの親和性を亢進する抗ヒトアデノシンA2a受容体モノクローナル抗体2012

    • Inventor(s)
      小笠原諭、日野智也
    • Industrial Property Rights Holder
      独立行政法人科学技術振興機構、島村達郎(他5名)
    • Industrial Property Number
      特願2012-49812
    • Filing Date
      2012-03-06

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Published: 2014-09-25  

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