Study on cell polarity regulation in plant epidermis

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: TANAKA Hirokazu 大阪大学, 理学(系)研究科(研究院), 助教 (10589922)
• Project Period (FY): 2011 – 2013
• Keywords: 植物 / 細胞・組織 / 発生・分化

Research Abstract

During plant growth and development, various functional proteins exhibit asymmetric localization at the plasma membrane and play critical roles in regulating cellular communications and cell function. PEL1 protein asymmetrically localizes preferentially at the outer sides of shoot epidermal cells and is involved in cuticle formation. However, very little is known about the regulatory mechanisms underlying the polar localization. We aimed to reveal the regulatory mechanism underlying this process and performed a forward genetic mutant screen using an EMS-mutagenized PEL1-GFP population. We have identified several candidate mutants, which were defective in cuticle-related defect. We expect that, by characterizing PEL1-GFP localization in these mutants, novel mutants with epidermal polarity defect might be identified.

Research Products

• [Journal Article] BEX1/ARF1A1C is required for BFA-sensitive recyc1ing of PIN auxin transporters and auxin-mediated deve1opment in Arabidopsis (2014)
  • Author(s): Tanaka,H.,Nodzynski,T.,Kitakura,S.,Feraru,M.I.,Sasabe,M.,Ishikawa,T.,K1eine-Vehn,J.,Kakimoto,T.,Frim1,J
  • Journal Title: Plant Cell Physiol Volume: 55 Pages: 737-749
  • DOI: 10.1093/pcp/pct196
  • Peer Reviewed
• [Journal Article] Ce11 po1arity and patterning by PIN trafficking through ear1y endosoma1 compartments in Arabidopsis thaliana (2013)
  • Author(s): Tanaka,H.,Kitakura,S.,Rakusova,H.,Uemura,T.,Feraru,M.I.,De Ricke,R.,Robert,S.,Kakimoto,T.,Frim1,J
  • Journal Title: PLOS Genetics Volume: 9 Pages: e1003540
  • DOI: 10.1371/journa1.pgen.1003540
  • Peer Reviewed
• [Presentation] シロイヌナズナのBEX1/ARF1A1Cはオーキシン排出タンパク質PINの細胞膜へのリサイクリングと発生制御に必要である (2014)
  • Author(s): 田中博和、Tomasz Nodzynski、北倉左恵子、石川朋美、柿本辰男、Jiri Friml
  • Organizer: 第55回植物生理学会年会
  • Place of Presentation: 富山大学(富山県)
  • Year and Date: 2014-03-18
• [Presentation] シロイヌナズナのSec1/Munc18タンパク質であるVPS45は細胞極性の制御に関与する (2013)
  • Author(s): 田中博和、北倉左恵子、Hana Rakusova,植村知博、柿本辰男、Jiri Friml
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)(ポスター発表)
  • Year and Date: 2013-12-03
• [Presentation] シロイヌナズナのARF1とARF GEF変異体の遺伝学的解析 (2013)
  • Author(s): 石川朋美、柿本辰男、田中博和
  • Organizer: 第36回分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)(ポスター発表)
  • Year and Date: 2013-12-03
• [Presentation] Ear1y endosoma1 components are required for po1ar PIN protein 1oca1ization and p1ant architecture in Arabidopsis (2013)
  • Author(s): Hirokazu Tanaka,Saeko Kitakura,Hana Rakusova,Tomohiro Uemura,Mugure1 I. Feraru,Riet De Rycke,Stephanie Robert,Tatsuo Kakimoto,Ji?i Friml
  • Organizer: 22th Internationa1 Conference on Arabidopsis Research
  • Place of Presentation: Sydney,Australia (poster presentation)
  • Year and Date: 2013-06-24
• [Presentation] 初期エンドソームに局在する輸送因子の細胞極性と発生パターンにおける役割 (2013)
  • Author(s): 田中博和、北倉左恵子、Hana Rakusova,Riet De Rycke,Stephanie Robert、柿本辰男、Ji?i Friml
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学津島キャンパス(岡山県)
  • Year and Date: 2013-03-22
• [Presentation] Deve1opmenta1 ro1es of ear1y endosoma1 components in Arabidopsis (2012)
  • Author(s): Hirokazu Tanaka
  • Organizer: The 4th NIBB-MPIPZ-TLL Symposium"Arabidopsis and Emerging Mode1 Systems"
  • Place of Presentation: Okazaki,Japan (poster presentation)
  • Year and Date: 2012-11-20
• [Remarks]
  • URL: http://www.bio.sci.osaka-u.ac.jp/~hirokazu.tanaka/index.htm1