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2013 Fiscal Year Final Research Report

Study on cell polarity regulation in plant epidermis

Research Project

  • PDF
Project/Area Number 23770147
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Functional biochemistry
Research InstitutionOsaka University

Principal Investigator

TANAKA Hirokazu  大阪大学, 理学(系)研究科(研究院), 助教 (10589922)

Project Period (FY) 2011 – 2013
Keywords植物 / 細胞・組織 / 発生・分化
Research Abstract

During plant growth and development, various functional proteins exhibit asymmetric localization at the plasma membrane and play critical roles in regulating cellular communications and cell function. PEL1 protein asymmetrically localizes preferentially at the outer sides of shoot epidermal cells and is involved in cuticle formation. However, very little is known about the regulatory mechanisms underlying the polar localization. We aimed to reveal the regulatory mechanism underlying this process and performed a forward genetic mutant screen using an EMS-mutagenized PEL1-GFP population. We have identified several candidate mutants, which were defective in cuticle-related defect. We expect that, by characterizing PEL1-GFP localization in these mutants, novel mutants with epidermal polarity defect might be identified.

  • Research Products

    (9 results)

All 2014 2013 2012 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results) Remarks (1 results)

  • [Journal Article] BEX1/ARF1A1C is required for BFA-sensitive recyc1ing of PIN auxin transporters and auxin-mediated deve1opment in Arabidopsis2014

    • Author(s)
      Tanaka,H.,Nodzynski,T.,Kitakura,S.,Feraru,M.I.,Sasabe,M.,Ishikawa,T.,K1eine-Vehn,J.,Kakimoto,T.,Frim1,J
    • Journal Title

      Plant Cell Physiol

      Volume: 55 Pages: 737-749

    • DOI

      10.1093/pcp/pct196

    • Peer Reviewed
  • [Journal Article] Ce11 po1arity and patterning by PIN trafficking through ear1y endosoma1 compartments in Arabidopsis thaliana2013

    • Author(s)
      Tanaka,H.,Kitakura,S.,Rakusova,H.,Uemura,T.,Feraru,M.I.,De Ricke,R.,Robert,S.,Kakimoto,T.,Frim1,J
    • Journal Title

      PLOS Genetics

      Volume: 9 Pages: e1003540

    • DOI

      10.1371/journa1.pgen.1003540

    • Peer Reviewed
  • [Presentation] シロイヌナズナのBEX1/ARF1A1Cはオーキシン排出タンパク質PINの細胞膜へのリサイクリングと発生制御に必要である2014

    • Author(s)
      田中博和、Tomasz Nodzynski、北倉左恵子、石川朋美、柿本辰男、Jiri Friml
    • Organizer
      第55回植物生理学会年会
    • Place of Presentation
      富山大学(富山県)
    • Year and Date
      2014-03-18
  • [Presentation] シロイヌナズナのSec1/Munc18タンパク質であるVPS45は細胞極性の制御に関与する2013

    • Author(s)
      田中博和、北倉左恵子、Hana Rakusova,植村知博、柿本辰男、Jiri Friml
    • Organizer
      第36回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)(ポスター発表)
    • Year and Date
      2013-12-03
  • [Presentation] シロイヌナズナのARF1とARF GEF変異体の遺伝学的解析2013

    • Author(s)
      石川朋美、柿本辰男、田中博和
    • Organizer
      第36回分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド(兵庫県)(ポスター発表)
    • Year and Date
      2013-12-03
  • [Presentation] Ear1y endosoma1 components are required for po1ar PIN protein 1oca1ization and p1ant architecture in Arabidopsis2013

    • Author(s)
      Hirokazu Tanaka,Saeko Kitakura,Hana Rakusova,Tomohiro Uemura,Mugure1 I. Feraru,Riet De Rycke,Stephanie Robert,Tatsuo Kakimoto,Ji?i Friml
    • Organizer
      22th Internationa1 Conference on Arabidopsis Research
    • Place of Presentation
      Sydney,Australia (poster presentation)
    • Year and Date
      2013-06-24
  • [Presentation] 初期エンドソームに局在する輸送因子の細胞極性と発生パターンにおける役割2013

    • Author(s)
      田中博和、北倉左恵子、Hana Rakusova,Riet De Rycke,Stephanie Robert、柿本辰男、Ji?i Friml
    • Organizer
      第54回日本植物生理学会年会
    • Place of Presentation
      岡山大学津島キャンパス(岡山県)
    • Year and Date
      2013-03-22
  • [Presentation] Deve1opmenta1 ro1es of ear1y endosoma1 components in Arabidopsis2012

    • Author(s)
      Hirokazu Tanaka
    • Organizer
      The 4th NIBB-MPIPZ-TLL Symposium"Arabidopsis and Emerging Mode1 Systems"
    • Place of Presentation
      Okazaki,Japan (poster presentation)
    • Year and Date
      2012-11-20
  • [Remarks]

    • URL

      http://www.bio.sci.osaka-u.ac.jp/~hirokazu.tanaka/index.htm1

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Published: 2015-06-25  

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