2012 Fiscal Year Final Research Report
Functional analyses of mammalian translocon of the ER
| Project/Area Number |
23770151
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | University of Hyogo |
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Principal Investigator |
KIDA Yuichiro 兵庫県立大学, 大学院・生命理学研究科, 助教 (10423899)
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| Research Collaborator |
SAKAGUCHI Masao 兵庫県立大学, 大学院・生命理学研究科, 教授 (30205736)
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| Project Period (FY) |
2011 – 2012
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| Keywords | タンパク質膜透過 / シグナル配列 / トランスロコン / 小胞体 / 膜タンパク質 |
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| Research Abstract |
(1) Marginally hydrophobic sequences, which cannot be integrated into the membrane, can be recognized by the ER translocon. We are now working toward identification of the recognition site of hydrophobic sequences in the Sec61 channel. (2) Novel integration mode of transmembrane sequences of a multi-spanning membrane protein is actually working in the membrane integration via the ER translocon. (3) The details of the translocation-deceleration effect by positively charged residues in the translocating polypeptide chain were determined. (4) The effects of cholesterol and phosphatidylserine for protein translocation and integration via the ER translocon were found.
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