2013 Fiscal Year Final Research Report
The regulation of intracellular lysophospholipase D activity
| Project/Area Number |
23770153
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Dokkyo Medical University |
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Principal Investigator |
AOYAMA Chieko 獨協医科大学, 医学部, 助教 (90420778)
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| Project Period (FY) |
2011 – 2012
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| Keywords | リゾホスホリパーゼD / リゾホスファチジン酸 / 三量体Gタンパク質 |
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| Research Abstract |
Lysophospholipase D (lysoPLD) is a key enzyme to produce bioactive phospholipid lysophosphatidic acid (LPA), which mediates multiple physiological and pathological events. We purified a novel lysoPLD from rat brain microsome and identified heterotrimeric G protein subunits G alpha q and G beta 1 in the purified fraction. In the present study, it is demonstrated that the other type of G alpha subunit, G alpha 12 also exhibit lysoPLD activity. From the FLAG tagged G alpha protein mutant study, it is suggested that the G protein requires to form a complex for its lysoPLD activity.
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