2012 Fiscal Year Final Research Report
Analyses of the interaction between glycolipids and membrane receptor by novel optics technology
| Project/Area Number |
23770155
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokai University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | イメージング / 糖脂質 / マイクロドメイン / 膜受容体 / ガングリオシド / 蛍光顕微鏡 / 成長因子受容体 / 糖鎖 / 生細胞イメージング |
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| Research Abstract |
Lipid bilayer is an elemental structural component of the cell membrane. Latest researches show that various lipid molecules are not distributed disorderly and we have recognized it as "lipid microdomain". Lipid microdomains are dynamic structures constantly in the process of either association or dissociation . It has been suggested that these microdomains enriched in cholesterol and sphingolipids could play an important rule in many cellular processes including signal transduction, membrane trafficking , cytoskeletal organization, and pathogen entry. In my laboratory, we study about the mechanism which composition change of lipid microdomain influence localization and signaling of membrane receptors. By novel optics technology such as fluorescence correlation spectroscopy (FCS) and fluorescence recovery after photobleaching (FRAP), we are trying to quantify this interaction and elucidate the rule of lipid microdomain.
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