Studying on ppGalNAcT with focus on the roles of mucin-type carbohydrates in the retinal development.

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Functional biochemistry
• Research Institution: Kyoto Sangyo University
• Principal Investigator: NAKAYAMA Yoshiaki 京都産業大学, 総合生命科学部, 助教 (40512455)
• Co-Investigator(Renkei-kenkyūsha): KUROSAKA Akira 京都産業大学, 総合生命科学部, 教授 (90186536) ; NAKAMURA Naosuke 京都産業大学, 総合生命科学部, 講師 (30424964) ; ITOH Nobuyuki 京都大学, 薬学研究科, 教授 (10110610) ; MIYAKE Ayumi 京都大学, 薬学研究科, 講師 (40346044)
• Project Period (FY): 2011 – 2012
• Keywords: 糖鎖 / 神経発生 / ゼブラフィッシュ / ムチン

Research Abstract

We cloned and identified 18 genes encoding ppGalNAcT from zebrafish cDNA library, corresponding to 20 isozymes in human genome. Then, we detected their mRNAs in the embryos during early developmental stages, and found that each of members has characteristic expression patterns mainly in eye, brain and skeletal muscle. Suppression of ppGalNAcT17 and T18 using antisense RNA led to developmental defects in the central nervous system and tail, respectively.

Research Products

• [Journal Article] Functions of MAPR (membrane-associated progesterone recep-tor) family members as heme/steroid-binding proteins. (2012)
  • Author(s): I. Kimura, Y. Nakayama, M. Konishi, K. Terasawa, M. Ohta, N. Itoh, and M. Fujimoto
  • Journal Title: Curr. Protein Pept. Sci. Volume: 13 Pages: 687-696
  • DOI: DOI:10.2174/1389203711209070687
  • Peer Reviewed
• [Journal Article] A putative polypeptide N-acetylgalactosaminyltrans-ferase/Williams-Beuren syndrome chromosome region 17 (WBSCR17) regulates lamellipodium formation and macropinocytosis. (2012)
  • Author(s): Y. Nakayama, N. Nakamura, S. Oki, M. Wakabayashi, Y. Ishihama, A. Miyake, N. Itoh, and A. Kurosaka
  • Journal Title: J. Biol. Chem. Volume: 287 Pages: 32222-32235
  • DOI: DOI:10.1074/jbc.M112.370932
  • Peer Reviewed
• [Journal Article] Neucrin, a novel secreted antagonist of canonical Wnt signaling, plays roles in developing neural tissues in zebrafish. (2012)
  • Author(s): A. Miyake, S. Nihno, Y. Murakoshi, A. Satsuka, Y. Nakayama, and N. Itoh
  • Journal Title: Mech. Dev. Volume: 128 Pages: 577-590
  • DOI: DOI:10.1016/j.mod.2012.01.001
  • Peer Reviewed
• [Presentation] ゼブラフィッシュ初期発生におけるポリペプチド-Nアセチルガラクトサミン転移酵素の機能解析 (2012)
  • Author(s): T.Kawai
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
  • Year and Date: 2012-12-13
• [Presentation] Biological roles of a brain-specific polypeptide GalNAc-transferase in zebrafish (2012)
  • Author(s): A. Kurosaka
  • Organizer: The 5th Asian Federation of Laboratory Animal Science Associations Congress
  • Place of Presentation: Bangkok (Thailand)
  • Year and Date: 2012-10-11
• [Presentation] Roles of mucin-type carbohydrates in endocytosis (2012)
  • Author(s): Y. Nakayama
  • Organizer: 2012 Joint Seminar in Science and Technology
  • Place of Presentation: Bangkok (Thailand)
  • Year and Date: 2012-03-15
• [Presentation] A putative polypeptide GalNAc transferase, WBSCR17, regulates endocytosis in HEK293T cells (2011)
  • Author(s): Y. Nakayama
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2011-12-14
• [Presentation] Developmental roles of putative polypeptide GalNAc-transferases in zebrafish (2011)
  • Author(s): N. Nakamura
  • Organizer: 2011 Annual Conference ofthe Society for Glycobiology
  • Place of Presentation: Seattle (USA)
  • Year and Date: 2011-11-11
• [Presentation] The biological roles of a putative polypeptide GalNAc-transferase/WBSCR17 (2011)
  • Author(s): Y. Nakayama
  • Organizer: Glyco21
  • Place of Presentation: Vienne(Austria)
  • Year and Date: 2011-08-22
• [Book] Genetic Disorders (2013)
  • Author(s): Y. Nakayama, N. Nakamura, D. Tsuji, K. Itoh, and A. Kurosaka
  • Total Pages: 243-269
  • Publisher: Genetic deseases associated with glycosylation disorders in mammalian glycoproteins.
  • URL: http://www.intechopen.com/books/genetic-disorders/genetic-diseases-associated-with-protein-glycosylation-disorders-in-mammals
• [Book] Intech, Human Genetic Diseases, (2011)
  • Author(s): Konishi, A. Miyake, and N. Itoh
  • Total Pages: 21
  • Publisher: The FGFFamily in Humans, Mice, and Zebrafish:Development, Physiology, and Patho-physiology.
  • URL: http://www.intechopen.com/books/human-genetic-diseases/the-fgf-family-inhumans-mice-and-zebrafish-developmentphysiology-and-pathophysiology
• [Remarks]
  • URL: http://www.cc.kyoto-su.ac.jp/~kurosaka/