Measurement of cellular dynamics using digital image correlation method

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770167
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biophysics
• Research Institution: Hokkaido University
• Principal Investigator: MIZUTANI Takeomi 北海道大学, 先端生命科学研究科(研究院), 助教 (40451405)
• Project Period (FY): 2011 – 2013
• Keywords: バイオイメージング

Research Abstract

Using live cell imaging and digital image correlation method, we measured cytoskeletal dynamics. This method will be helpful to study mechanical interaction between cytoskeletons or cellular contractile force in 3D.

Research Products

• [Journal Article] Modulation of extracellular conditions prevents the multilayering of the simple epithelium (2014)
  • Author(s): T. Mizutani, K. Takeda, H. Haga, M. Todo, K. Kawabata
  • Journal Title: Histochemistry and Cell Biology Volume: Vol. 141(5) Pages: 459-471
  • DOI: 10.1007/s00418-013-1176-8
  • Peer Reviewed
• [Journal Article] Tempo-Spatial Change of Cellular Stiffness and Geometry in the Process of Developing Epithelial Cell-Cell Adhesion Measured by Atomic Force Microscopy (2013)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Journal Title: Japanese Journal of Applied Physics Conference Proceedings Volume: Vol. 1 Pages: 011004(7)
  • DOI: 10.1143/JJAPCP.1.011004
  • Peer Reviewed
• [Journal Article] Irradiation-tolerant lung cancer cells acquire invasive ability dependent on dephosphorylation of the myosin regulatory light chain (2013)
  • Author(s): S. Ishihara, M. Yasuda, T. Nishioka, T. Mizutani, K. Kawabata, H. Shirato, H. Haga
  • Journal Title: FEBS Letter Volume: Vol. 587(6) Pages: 732-6
  • DOI: 10.1016/j.febslet.2013.01.055
  • Peer Reviewed
• [Journal Article] The number of cyclic stretch regulates cellular elasticity in C2C12 myoblasts (2012)
  • Author(s): K. Takemoto, T. Mizutani, K. Tamura, K. Takeda, H. Haga, K. Kawabata
  • Journal Title: CellBio Volume: Vol. 1(1) Pages: 1-10
  • DOI: 10.4236/cellbio.2012.11001
  • Peer Reviewed
• [Journal Article] Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice (2012)
  • Author(s): N. Seito, T. Yamashita, Y. Tsukuda, Y. Matsui, A. Urita, T. Onodera, T. Mizutani, H. Haga, N. Fujitani, Y. Shinohara, A. Minami, N. Iwasaki
  • Journal Title: rthritis & Rheumatism Volume: Vol. 64(8) Pages: 2579-88
  • DOI: 10.1002/art.34463
  • Peer Reviewed
• [Journal Article] 2次元と3次元環境下での細胞と細胞骨格の力学計測 (2012)
  • Author(s): 水谷武臣
  • Journal Title: M&M2012材料力学カンファレンス論文集 Pages: OS1101
• [Journal Article] Active fluctuation in the cortical cytoskeleton observed by high-speed live-cell scanning probe microscopy (2011)
  • Author(s): K. Tamura, T. Mizutani, H. Haga, K Kawabata
  • Journal Title: Acta Biomaterialia Volume: Vol. 7(10) Pages: 3766-3772
  • DOI: 10.1016/j.actbio.2011.06.013
  • Peer Reviewed
• [Presentation] 細胞の力学コミュニケーション (2013)
  • Author(s): 水谷武臣
  • Organizer: 大阪府立母子保健総合医療センター研究所セミナー
  • Place of Presentation: 大阪府立母子保健総合医療センター研究所(和泉市)
  • Year and Date: 2013-11-06
• [Presentation] Tempo-spatial Change of Cellular Stiffness in the Process of Developing Epithelial Cell-cell Adhesion Measured by Atomic Force Microscopy (2012)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Organizer: 20th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Okinawa Kariyushi Urban Resort Naha(那覇市)
  • Year and Date: 2012-12-17
• [Presentation] 2次元と3次元環境下での細胞と細胞骨格の力学計測 (2012)
  • Author(s): 水谷武臣
  • Organizer: M&M2012材料力学カンファレンス
  • Place of Presentation: 愛媛大学(松山市)
  • Year and Date: 2012-09-22
• [Presentation] Evaluation of microtubule deformation by live cell imaging and image analysis (2012)
  • Author(s): R. Tanaka, T. Mizutani, H. Haga, K. Kawabata
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学(名古屋市)
  • Year and Date: 2012-09-22
• [Presentation] 画像解析を用いた細胞内外の力学量計測 (2012)
  • Author(s): 水谷武臣
  • Organizer: 第四回「光塾」
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2012-08-26
• [Presentation] 細胞骨格に発生した不均一な歪み : ライブセルイメージングと画像解析による力学量測定法の提案 (2012)
  • Author(s): 田中良昌、水谷武臣、芳賀永、川端和重
  • Organizer: 2011年度日本生物物理学会北海道支部例会
  • Place of Presentation: 旭川市民文化会館(旭川市)
  • Year and Date: 2012-03-06
• [Presentation] Imaging for Cellular Mechanics : Dynamic Behavior of Cytoskeletal Networks and Cellular Contractile Force (2011)
  • Author(s): T. Mizutani
  • Organizer: The Fifth Shanghai International Conference on Biophysics and Molecular Biology
  • Place of Presentation: Huashia Hotel(China)
  • Year and Date: 2011-11-05
• [Presentation] 細胞の時空間ダイナミクス計測 : ライブセルイメージングから細胞骨格の力学バランスと細胞が発生する力をみる (2011)
  • Author(s): 水谷武臣
  • Organizer: 九州大学共同利用研究会
  • Place of Presentation: 九州大学(福岡市)
  • Year and Date: 2011-09-21
• [Remarks]
  • URL: http://altair.sci.hokudai.ac.jp/g3/index.html