2012 Fiscal Year Final Research Report
Statistical analysis of flagella beating by 3-D tracking microscopy
| Project/Area Number |
23770172
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
YAMANO Takashi 京都大学, 大学院・生命科学研究科, 助教 (70570167)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 運動・輸送 / クラミドモナス / 三次元トラッキング顕微鏡 |
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| Research Abstract |
We developed 3D-tracking microscopy by a new image processing algorithm to achieve high-speed autofocusing of Chlamydomonas reinhardtii, a motile green alga. Using this algorithm and high-speed visual feedback, we could pursue a single Chlamydomonas cell to keep it at the center of the field and depth of view, and visualize the two flagella continuously along with tracking it at high spatiotemporal resolution.
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