2012 Fiscal Year Final Research Report
Stimulation-secretion coupling of artificial secretory cells using cell-sized proteoliposomes
| Project/Area Number |
23770181
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Nagoya City University |
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Principal Investigator |
TADOKORO Satoshi 名古屋市立大学, 大学院・薬学研究科, 助教 (20389109)
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| Project Period (FY) |
2011 – 2012
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| Keywords | SNARE / 開口放出 / 人工細胞 / リポソーム |
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| Research Abstract |
Mast cells are involved in allergic responses. Antigen stimulation causes elevation of intracellular Ca2+ concentration, which triggers exocytotic release of inflammatory mediators. Therefore, it is important for the development of new anti-allergic drugs to elucidate the mechanism of exocytosis. In this study, we found that in addition to Ca2+, synaptotagmin2, which is a Ca2+ sensor for mast cell exocytosis enhanced exocytotic membrane fusion mediated by phosphatidylserine. These results indicated that not only proteins and Ca2+ but phospholipid are involved in mast cellexocytosis.
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