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2012 Fiscal Year Final Research Report

Tracking of the bacterial flagellar rotation using single fluorescent microscopy

Research Project

  • PDF
Project/Area Number 23770183
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionHosei University

Principal Investigator

SOWA Yoshiyuki  法政大学, 生命科学部, 講師 (10519440)

Project Period (FY) 2011 – 2012
Keywords細菌べん毛モーター
Research Abstract

The bacterial flagellar motor is a reversible rotary nano-machine, which is powered by ion flux across the cell membrane. The motor has several interesting features as fast rotation, regulation system of torque generation and so on. Their rotation, however, have been measured via flagellar filaments extend from cell body, rather than via the motor basal body directly. To track its rotation directly at molecular level, we have developed single molecule fluorescent microscopy and methods to label the motor basal body.

  • Research Products

    (21 results)

All 2013 2012 2011 Other

All Journal Article (1 results) Presentation (17 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Microscopic analysis of bacterial motility at high pressure2012

    • Author(s)
      Masayoshi Nishiyama & Yoshiyuki Sowa.
    • Journal Title

      Biophys.J

      Volume: 102 Pages: 1872-1880

    • DOI

      DOI:10.1016/j.bpj.2012.03.033

  • [Presentation] 液体ハンドリングのみによって行う微生物ゲノムの連続編集2013

    • Author(s)
      冨永将大,嶋村陽,曽和義幸,川岸郁朗,斎藤恭一,梅野太輔
    • Organizer
      第6回日本ゲノム微生物学会年会
    • Place of Presentation
      長浜バイオ大学(滋賀県)
    • Year and Date
      20130310-0312
  • [Presentation] Dynamicconformationalchangesofflagellarfilamentobservedbyhigh-pressuremicroscopy2013

    • Author(s)
      Nishiyama,M.&Sowa,Y.
    • Organizer
      57thAnnualMeetingofBiophysicalSociety
    • Place of Presentation
      Philadelphia(アメリカ)
    • Year and Date
      2013-02-06
  • [Presentation] Microscopic analysis of bacterial motility at high pressure (Platform)2012

    • Author(s)
      M. Nishiyama and Y. Sowa.
    • Organizer
      56th Annual Meeting of Biophysical Society
    • Place of Presentation
      Boston アメリカ
    • Year and Date
      20120225-0229
  • [Presentation] A new setup for tracking of bacterial flagellar rotation in3-dimensionally2012

    • Author(s)
      Sowa, Y.
    • Organizer
      2012 Gordon ResearchConference on Sensory Transduction in Microorganisms
    • Place of Presentation
      Ventura (アメリカ)
    • Year and Date
      20120115-0121
  • [Presentation] 高圧力下にあるバク テリア遊泳運動の阻害機構2012

    • Author(s)
      西山雅祥,曽和義幸
    • Organizer
      2012 年 生体運 動研究合同班会議
    • Place of Presentation
      筑波大学 (茨城県)
    • Year and Date
      20120106-0108
  • [Presentation] Bacterial motility measured by a high-pressure microscope2012

    • Author(s)
      Nishiyama, M. & Sowa, Y.
    • Organizer
      7th International Conference on High Pressure Bioscience and Biotechnology (HPBB2012)
    • Place of Presentation
      ピアザ淡海 (滋 賀県)
    • Year and Date
      2012-10-31
  • [Presentation] Exploring the mechanism underlying sensing of the repellent Ni2+ by the aspartate chemoreceptor Tar of Escherichia coli2012

    • Author(s)
      Inui, T., Tajima, H., Sowa, Y. & Kawagishi, I.
    • Organizer
      第50回生物物理学会年 会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-24
  • [Presentation] Control of bacterial flagellar rotation via crosstalk from a non-cognate histidine kinase to the response regulator CheY2012

    • Author(s)
      Umemura, T., Hara, C., Sowa, Y. & Kawagishi, I.
    • Organizer
      第50回生物物理 学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-24
  • [Presentation] Intracellular dynamics of xenobiotic efflu2012

    • Author(s)
      Yamamoto, K., Inaba, T., Sowa, Y. & Kawagishi, I.
    • Organizer
      x proteins in Escherichia coli. 第50回生物物理学会年会
    • Place of Presentation
      名古屋大 学 (愛知県)
    • Year and Date
      2012-09-24
  • [Presentation] Imaging of fluorescently-tagged motor components of bacterial flagella2012

    • Author(s)
      Sowa, Y. & Che, Y-S.
    • Organizer
      第50回生物物理学会 年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-23
  • [Presentation] Rotational measurement of Na+-driven chimeric flagellar motor with tandem PomA2012

    • Author(s)
      Che, Y-S & Sowa, Y.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-23
  • [Presentation] Dynamic conformational changes of flagellar filament observed by high-pressure microscopy2012

    • Author(s)
      Nishiyama, M. & Sowa, Y.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-22
  • [Presentation] Sodium dynamics of the bacterial flagellar motor2012

    • Author(s)
      Lo, C-J., Sowa, Y., Pilizota, T. & Berry, R.
    • Organizer
      第50回生物物理学会年会
    • Place of Presentation
      名古屋大学 (愛知県)
    • Year and Date
      2012-09-22
  • [Presentation] 高圧力下にあるバクテリア運動能の顕微解析2011

    • Author(s)
      西山雅祥,曽和義幸.
    • Organizer
      日本生物物理学会 第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Year and Date
      20110916-18
  • [Presentation] Na+駆動型キメラべん毛モーターにおける固定子PomA融合タンデムダイマーの機能解析2011

    • Author(s)
      蔡栄淑, 曽和義幸.
    • Organizer
      日本生物物理学会 第49回生物物理学会年会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Year and Date
      20110916-0918
  • [Presentation] Three-dimensinal tracking of bacterial flagellar rotation2011

    • Author(s)
      Sowa, Y.
    • Organizer
      日本生物物理学会
    • Place of Presentation
      兵庫県立大学 (兵庫県)
    • Year and Date
      2011-09-16
  • [Presentation] 大腸菌走 化性応答のビデオ解析2011

    • Author(s)
      藤畑将理, 高橋浩一, 曽和義幸.
    • Organizer
      21 世紀大腸菌研究 会
    • Place of Presentation
      ホテル木曽路 (長野県)
    • Year and Date
      2011-05-19
  • [Book] ComprehensiveBiphysics AcademicPress2012

    • Author(s)
      YoshiyukiSowa&RichardM.Berry
    • Total Pages
      50-71
    • Publisher
      TheRotaryBacterialFlagellarMotor
  • [Remarks]

    • URL

      http://sowalab.ws.hosei.ac.jp

  • [Patent(Industrial Property Rights)] 高速かつ高効率なゲノム改変法2012

    • Inventor(s)
      梅野太輔,冨永将大,田代洋平,川 岸郁朗,曽和義幸,稲葉岳彦,蔡栄淑
    • Industrial Property Rights Holder
      国立大学法人 千葉大学, 学校法人 法政大学
    • Industrial Property Number
      特願 2012-113083
    • Filing Date
      2012-05-17

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Published: 2014-09-25  

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