2012 Fiscal Year Final Research Report
Development of real-time secretion analysis of immune single cells
| Project/Area Number |
23770189
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SHIRASAKI Yoshitaka 独立行政法人理化学研究所, 免疫ゲノミクス研究グループ, 研究員 (70469948)
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| Project Period (FY) |
2011 – 2012
|
| Keywords | 単一細胞解析 / イムノアッセイ / 分泌測定 / マイクロウェル |
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| Research Abstract |
We developed a platform for real-time secretion analysis at the single cell resolution. The platform enabled to monitor secretion dynamics of thousand of single cells with 30-min time resolution for over 8 hours. We also demonstrated multi-parametric measurement of secretion dynamics and cellular states with 1-min time resolution. We applied our platform to inflammatory responses of immune cells and observed intercellular and intracellular heterogeneity of cytokine secretion.
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Research Products
(12 results)
