2012 Fiscal Year Final Research Report
Analysis of mechanisms of translational control using transgenes and visualization of the site and timing of translation
| Project/Area Number |
23770196
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
KOTANI Tomoya 北海道大学, 大学院・理学研究院, 准教授 (70419852)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 翻訳 / mRNA局在 / シス因子 / コード領域 / 卵母細胞 |
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| Research Abstract |
Localization and translational control of cytoplasmic mRNAs play key roles in the regulation of site and timing of gene expression. We analyzed molecular mechanisms by which localization and translational control of cyclin B1 mRNA deposited in oocytes are regulated using transgenes and real-time imaging of the site and timing of translation. In this study, we revealed that a cis-acting element consisting of 9 nucleotides sequences in the coding region is necessary for the regulation of both localization and translational control of the mRNA. Our results suggest a functional link between mRNA localization and translational control.
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