2012 Fiscal Year Final Research Report
Role of RBM42 in proliferation and survival
| Project/Area Number |
23770199
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Keywords | RNA結合タンパク質 |
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| Research Abstract |
Post-transcriptional regulation of gene expression by RNA-binding proteins has pivotal roles in many biological processes. We analyzed role of two RNA-binding proteins, RBM42 and hnRNPK, in cell proliferation. In RBM42 and hnRNPK knockdown cells, G1/S progression was delayed as compared to control cells. E2F is an essential transcription factor that is required for gene expression in G1/S transition. The expression of E2F target genes was significantly reduced in RBM42 and hnRNPK knockdown cells. Furthermore, we found that hnRNPK associates with E2F mRNA and regulates the mRNA stability of E2F mRNA. These results suggest that hnRNPK positively regulates cell cycle progression through the regulation of E2F mRNA stability.
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