Mechanism of protein synthesis in mammalian mitochondria

2012 Fiscal Year Final Research Report

• Project/Area Number: 23770202
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Molecular biology
• Research Institution: The University of Tokyo
• Principal Investigator: TOMITA Nono (TAKEUCHI Nono) 東京大学, 大学院・新領域創成科学研究科, 准教授 (80323450)
• Project Period (FY): 2011 – 2012
• Keywords: 蛋白質合成 / ミトコンドリア

Research Abstract

We have obtained following results on the mechanism of protein synthesis in mammalian mitochondrial. (1) The role of GTP-hydrolysis during ribosome recycling process has been clarified. (2) The sturucture of 55S ribosome has been determined at 10 A resolution by cryoEM reconstitution. The E-site tRNA on 55S ribosome has been visualized. (3) The ribosome binding properties of the mitochondrial ribosome biogenesis factor (ObgH1, Mtg1) have been clarified.

Research Products

• [Journal Article] Human G-proteins, ObgH1 and Mtg1, associate with the large mitochondrial ribosome subunit and are involved in translation and assembly of respiratory complexes. (2013)
  • Author(s): Kotani T., Akabane S., Takeyasu K., Ueda T. and Takeuchi N.
  • Journal Title: Nucleic Acids Res. Volume: 41 Pages: 3713-22
  • DOI: DOI:10.1093/nar/gkt079
  • Peer Reviewed
• [Journal Article] Possible steps ofcomplete disassembly of post-termination complex by yeast eEF3 deduced from inhibition by translocation inhibitors. (2012)
  • Author(s): Kurata S., Shen B., Liu JO., Takeuchi N., Kaji A. and Kaji H.
  • Journal Title: Nucleic Acids Res. Volume: 41(1) Pages: 264-76
  • DOI: DOI:10.1093/nar/gks958
  • Peer Reviewed
• [Journal Article] RsfA (YbeB) Proteins Are Conserved Ribosomal Silencing Factors. (2012)
  • Author(s): Hauser R, Pech M, Kijek J, Yamamoto H, Titz B, Naeve F, Tovchigrechko A,Yamamoto K, Szaflarski W, Takeuchi N, Stellberger T, Diefenbacher ME, NierhausKH, Uetz P.
  • Journal Title: PLoS Genet. Volume: 8(7) Pages: e1002815
  • DOI: DOI:10.1371/journal.pgen.1002815
  • Peer Reviewed
• [Journal Article] Taurine-containing uridine modifications in tRNA anticodons arerequired to decipher non-universal genetic codes in ascidian mitochondria. (2011)
  • Author(s): Suzuki T., Miyauchi K., Suzuki T., YokoboriS., Shigi N., Kondow A., Takeuchi N., Yamagishi A., Watanabe K.
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 35494-8
  • DOI: DOI:10.1074/jbc.M111.279810
  • Peer Reviewed
• [Presentation] N-terminal extension of mammalian mitochondrial (2012)
  • Author(s): Nono Tomita-Takeuchi
  • Organizer: RRF alters the role of GTP-hydrolysis by EF-G in ribosome recycling Complex life of mRNA
  • Place of Presentation: EMBL, Heidelberg, Germany
  • Year and Date: 2012-10-09
• [Presentation] Mechanism of ribosome recycling in mammalian mitochondrialprotein synthesis (2012)
  • Author(s): 入江遼平
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2012-07-19
• [Presentation] Functional analysis of Mtg1 in mammalian mitochondrial ribosomebiogenesis (2012)
  • Author(s): 小谷哲也
  • Organizer: 日本RNA学会年会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2012-07-19
• [Presentation] Protein Synthesis System of Mammalian Mitochondria (2011)
  • Author(s): Nono Takeuchi
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2011-12-15