2013 Fiscal Year Final Research Report
Investigation of the restart mechanisms of stalled DNA replication forks.
| Project/Area Number |
23770208
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Oita University |
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Principal Investigator |
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| Project Period (FY) |
2012 – 2013
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| Keywords | DNA損傷・修復 |
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| Research Abstract |
Precise DNA replication is important for all organism. If DNA replication does not complete properly, these organisms cannot maintain their cellular metabolisms. Since DNA replication stalls by DNA damages causes of incomplete DNA replication, all oraganisms provide the repair of stalled DNA replication forks. Here we addressed to understand the mechanisms of stalled DNA replication forks in mammalian cells. We previously showed that the MUS81-structure specific endonuclease is involved in the repair of stalled replication forks. Therefore, we first investigated how MUS81 is regulated in DNA damage networks. Expectedly, ATR-dependent damage responses occur at first. Then MUS81-dependent DNA breakages were introduced. Following this, ATM-dependent responses occurred. This network is important to encourage the repair of stalled forks. In addition, we have done the screening of MUS81-independent pathways and found several candidates. We are now characterizing the biochemical functions of these candidate factors.
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