2012 Fiscal Year Final Research Report
Investigation of the novel activation mechanism of MRTF, a master transcriptional regulator of the actin cytoskeleton.
| Project/Area Number |
23770225
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
MORITA Tsuyoshi 大阪大学, 大学院・医学系研究科, 助教 (80403195)
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| Project Period (FY) |
2011 – 2012
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| Keywords | 細胞骨格 / 運動 / 転写因子 / ガン / 平滑筋 |
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| Research Abstract |
In this study, I investigated the novel activation mechanism of myocardin family proteins, myocardin and MRTF. The activity of MRTF is suppressed by interaction with actin. I identified Tb4 protein as an inhibitory factor of MRTF/actin interaction. myocardin also interacts with actin, but its affinity for actin is rather low. I demonstrated that actin-related protein 5 (Arp5), instead of actin, tightly bound to myocardin and suppressed its activity. In smooth muscle cells, Arp5 expression level is very low, resulting in high activity of myocardin.
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