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2012 Fiscal Year Final Research Report

Role of FBP17,an EFC/F-BAR protein, in phagocytic cupformation

Research Project

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Project/Area Number 23770228
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionKobe University

Principal Investigator

TSUJITA Kazuya  神戸大学, 大学院・医学研究科, 助教 (10457054)

Project Period (FY) 2011 – 2012
KeywordsF-BAR / ドメイン / アクチン重合
Research Abstract

By performing in vitro analysis, we directly showed the mechanism by which FBP17 regulates actin polymerization on the membrane tubules. Physiologically, it was found that FBP17 is recruited to phagocytic cup via its F-BAR domain and that this accumulation is critical for phagocytic cup formation. Furthermore, RNAi and its rescue experiments revealed that the assembly of FBP17 at the plasma membrane is dependent on its F-BAR domain, promoting actin polymerization at phagocytic cup membrane. These data suggest that invaginated membrane caused by FBP17 may function as a scaffold for actin polymerization.

  • Research Products

    (7 results)

All 2013 2012 2011 Other

All Journal Article (5 results) Presentation (2 results)

  • [Journal Article] Physicochemical analysis from real-time imaging of liposome tubulation reveals the characteristics of individual F-BAR domain proteins.2013

    • Author(s)
      Tanaka-Takiguchi Y, Itoh T, Tsujita K, Yamada S, Yanagisawa M, Fujiwara K, Yamamoto A, Ichikawa M, Takiguchi K
    • Journal Title

      Langmuir.

      Volume: 29 Pages: 328-336

  • [Journal Article] ARAP1 regulates the ring size of circular dorsal ruffles through Arf1 and Arf52012

    • Author(s)
      Hasegawa J, Tsujita K, Takenawa T, Itoh T.
    • Journal Title

      Molecular Biology of The Cell

      Volume: 23 Pages: 2481-2489

  • [Journal Article] Characterization of the EFC/F-BAR domain protein2011

    • Author(s)
      Uezu A, Umeda K, Tsujita K, Suetsugu S, Takenawa T, Nakanishi H
    • Journal Title

      CHO2.Genes to Cell

      Volume: 8 Pages: 868-878

  • [Journal Article] SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain2011

    • Author(s)
      Hasegawa J, Tokuda E, Tenno T, TsujitaK, Sawai H, Hiroaki H, Takenawa T, Itoh T.
    • Journal Title

      Journal of Cell Biology

      Volume: 193 Pages: 901-916

  • [Journal Article] Antagonistic regulation of F-BAR protein assemblies controls actin polymerization during podosome formation

    • Author(s)
      Tsujita K,Kondo A, Kurisu S, Hasegawa J, Itoh T, Takenawa T.
    • Journal Title

      Journal of Cell Science

  • [Presentation] Antagonistic regulation of F-BAR domain assembly on membrane.2011

    • Author(s)
      辻田 和也
    • Organizer
      第84回日本生化学会大会(口頭発表)
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-23
  • [Presentation] Regulation of membrane mediated actin assembly by EFC/F-BAR proteins.2011

    • Author(s)
      辻田 和也
    • Organizer
      第63回日本細胞生物学会大会(口頭発表)
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-29

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Published: 2014-09-25  

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