Mechanisms of size determination of neurogenic ectoderm

2013 Fiscal Year Final Research Report

• Project/Area Number: 23770241
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: University of Tsukuba
• Principal Investigator: YAGUCHI Shunsuke 筑波大学, 生命環境系, 准教授 (00505331)
• Project Period (FY): 2011 – 2012
• Keywords: 細胞分化 / 体軸形成

Research Abstract

The aim of this study is to understand the molecular mechanisms that determine the size of neurogenic ectoderm of the sea urchin embryos, especially focusing on the molecular interaction between signaling pathways that specify the cell fate along with the primary and secondary body axes. By loss-of-function experiments for each molecules, I could show that Wnt-JNK and Nodal pathways that are important to form primary and secondary axes, respectively, are essential for sea urchin embryos to maintain the precise size of anterior neurogenic ectoderm.

Research Products

• [Journal Article] Imaging neural development in embryonic and larval urchins (2014)
  • Author(s): Krupke O, Yaguchi S, Yaguchi J, Burke RD.
  • Journal Title: Methods in Molecular Biology Volume: 1128 Pages: 147-160
  • Peer Reviewed
• [Journal Article] Glutathion transferase theta in apical ciliary tuft regulates mechanical reception and swimming behavior of sea urchin embryos (2013)
  • Author(s): Jin, Y, Yaguchi S, Shiba K, Yamada L, Yaguchi J, Shibata D. Sawada H. Inaba K.
  • Journal Title: Cytosketlon Volume: 70 Pages: 453-470
  • Peer Reviewed
• [Journal Article] Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo (2012)
  • Author(s): Yaguchi J, Angerer LM, Inaba K, Yaguchi S.
  • Journal Title: Dev Biol Volume: 363 Pages: 74-83
  • Peer Reviewed
• [Journal Article] Fez function is required to maintain the size of the animal plate in the sea urchin embryo (2011)
  • Author(s): Yaguchi S, Yaguchi J, Wei Z, Jin Y, Angerer LM, Inaba K.
  • Journal Title: Development Volume: 138 Pages: 4233-4243
  • Peer Reviewed
• [Presentation] TGF-ß signals regulate the dorsoventral patterning of neurogenic ectoderm of the sea urchin embryo (2013)
  • Author(s): Yaguchi, J., Takeda, N., Inaba, K., Yaguchi, S.
  • Organizer: 46^<th> Annual meeting of the Japanese Society of Developmental Biologists
  • Place of Presentation: くにびきメッセ、松江
  • Year and Date: 2013-05-29
• [Presentation] TGF-ß signals regulate the dorsoventral patterning of neurogenic ectoderm of the sea urchin embryo (2012)
  • Author(s): Yaguchi, S.
  • Organizer: Developmental Biology of the Sea Urchin XXI
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2012-10-26
• [Presentation] Analysis of the apical tuft specific proteins with LC-MS/MS (2011)
  • Author(s): 金銀華、山田力志、澤田均、谷口俊介、稲葉一男
  • Organizer: 82^<nd> annual meeting of the Zoological Society of Japan
  • Place of Presentation: 旭川市神楽地区公共施設群、旭川市
  • Year and Date: 2011-09-21
• [Presentation] Homeobrain is required for the differentiation of serotonergic neurons in animal plate of the sea urchin embryos (2011)
  • Author(s): 谷口順子、竹田典代、稲葉一男、谷口俊介
  • Organizer: 82^<nd> annual meeting of the Zoological Society of Japan
  • Place of Presentation: 旭川市神楽地区公共施設群、旭川市
  • Year and Date: 2011-09-21
• [Presentation] Fez function is required for establishing a border between the neurogenic and non-neurogenic ectoderm (2011)
  • Author(s): 谷口俊介、谷口順子、Wei Zheng、金銀華、Angerer Lynne、稲葉一男
  • Organizer: 82^<nd> annual meeting of the Zoological Society of Japan
  • Place of Presentation: 旭川市神楽地区公共施設群、旭川市
  • Year and Date: 2011-09-21
• [Presentation] ウニ胚繊毛運動におけるCa^<2+>結合タンパク質カラクシンの機能 (2011)
  • Author(s): 水野克俊、谷口順子、柴小菊、谷口俊介、稲葉一男
  • Organizer: 82^<nd> annual meeting of the Zoological Society of Japan
  • Place of Presentation: 旭川市神楽地区公共施設群、旭川市
  • Year and Date: 2011-09-21
• [Presentation] Intracellular FoxQ2-Fez function is required for establishing a border between the neurogenic and non-neurogenic ectoderm (2011)
  • Author(s): Yaguchi, S, Yaguchi, J, Wei, Z, Jin, Y, Angerer, LM, Inaba, K.
  • Organizer: Developmental Biology of the Sea Urchin XX
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2011-04-28
• [Presentation] Dynamic changes in the borders of hbn expression in the animal pole domain of the sea urchin embryo are regulated by Wnt and TGF-ß signals (2011)
  • Author(s): Yaguchi, J., Takeda, N., Inaba, K., Yaguchi, S.
  • Organizer: Developmental Biology of the Sea Urchin XX
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2011-04-28
• [Presentation] Zinc finger homeobox is required for the differentiation of serotonergic neurons in the sea urchin embryo (2011)
  • Author(s): Yaguchi, J., Inaba, K., Angerer, RC, Angerer LM, Yaguchi, S.
  • Organizer: Developmental Biology of the Sea Urchin XX
  • Place of Presentation: Woods Hole, USA
  • Year and Date: 2011-04-28
• [Remarks]
  • URL: http://www.shimoda.tsukuba.ac.jp/~yaguchi/index.html