2013 Fiscal Year Final Research Report

Identification of the major gene for resistance to Soybean dwarf virus

Research Project

Project/Area Number	23780007
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Breeding science
Research Institution	Hokkaido Research Organization
Principal Investigator	YAMASHITA Yoko 地方独立行政法人北海道立総合研究機構, 農業研究本部中央農業試験場, 研究員 (30462386)
Project Period (FY)	2011 – 2013
Keywords	育種学 / 遺伝子 / ジーンサイレンシング
Research Abstract	The expression of the candidate Rsdv1 gene, the major Soybean dwarf virus (SbDV) resistance gene derived from the soybean cultivar 'Wilis', was suppressed by virus-induced gene silencing (VIGS). An evaluation method for SbDV resistance was developed, in which SbDV was detected by ELISA instead of symptom observation. This method enabled resistance evaluation of gene-silenced soybean lines whose symptoms of SbDV are not detectable due to the symptoms caused by VIGS. VIGS of the candidate Rsdv1 gene did not significantly change the percentage of SbDV-infected plants, suggesting that VIGS was not suitable for identification of Rsdv1, or that additional neighboring genes were necessary for resistance.