2013 Fiscal Year Final Research Report
Molecular dissection of species-specific transgene silencing
| Project/Area Number |
23780027
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Horticulture/Landscape architecture
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
MISHIBA Kei-ichiro 大阪府立大学, 生命環境科学研究科(系), 准教授 (70390888)
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| Project Period (FY) |
2011 – 2013
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| Keywords | DNAメチル化 / リンドウ / 分子育種 / レタス / サイレンシング / 遺伝子組換え / 園芸 |
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| Research Abstract |
Molecular mechanism of the 35S promoter-specific DNA methylation in gentian was investigated in this study. Two distinct de novo methylated regions were identified after the methylation analysis of an entire T-DNA segment in transgenic gentian. Both of the regions contain two consensus sequences, to which gentian nuclear extracts could bind, and yeast one-hybrid screening identified candidates for binding to the sequences. Additionally, relationship between DNA methylation and histone modification was investigated using transgenic gentian cell suspension culture, and the results showed that de novo methylation of the 35S region occurred in connection with histone modification. To investigate whether the 35S promoter-specific DNA methylation exists in other plant species, transgenic lettuce plants having 35S promoter were produced. As the result, suppression of the transgene expression and hypermethylation of the 35S promoter region were found in the transgenic lettuce plants.
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Research Products
(7 results)
